Heterodimeric antibodies that bind cd3 and psma

ABSTRACT

The present invention is directed to heterodimeric antibodies that bind CD3 and PSMA.

PRIORITY CLAIM

This application claims priority to U.S. Provisional Application Nos. 62/264,261, filed Dec. 7, 2015 and 62/324,823, filed Apr. 19, 2016 which are expressly incorporated by reference in their entireties.

TECHNICAL FIELD

The present disclosure relates to methods and compositions for the treatment of cancers (e.g., prostate cancer).

BACKGROUND OF THE INVENTION

Antibody-based therapeutics have been used successfully to treat a variety of diseases, including cancer and autoimmune/inflammatory disorders. Yet improvements to this class of drugs are still needed, particularly with respect to enhancing their clinical efficacy. One avenue being explored is the engineering of additional and novel antigen binding sites into antibody-based drugs such that a single immunoglobulin molecule co-engages two different antigens. Such non-native or alternate antibody formats that engage two different antigens are often referred to as bispecifics. Because the considerable diversity of the antibody variable region (Fv) makes it possible to produce an Fv that recognizes virtually any molecule, the typical approach to bispecific generation is the introduction of new variable regions into the antibody.

A number of alternate antibody formats have been explored for bispecific targeting (Chames & Baty, 2009, mAbs 1[6]:1-9; Holliger & Hudson, 2005, Nature Biotechnology 23[9]:1126-1136; Kontermann, mAbs 4(2): 182 (2012), all of which are expressly incorporated herein by reference). Initially, bispecific antibodies were made by fusing two cell lines that each produced a single monoclonal antibody (Milstein et al., 1983, Nature 305:537-540). Although the resulting hybrid hybridoma or quadroma did produce bispecific antibodies, they were only a minor population, and extensive purification was required to isolate the desired antibody. An engineering solution to this was the use of antibody fragments to make bispecifics. Because such fragments lack the complex quaternary structure of a full length antibody, variable light and heavy chains can be linked in single genetic constructs. Antibody fragments of many different forms have been generated, including diabodies, single chain diabodies, tandem scFv's, and Fab2 bispecifics (Chames & Baty, 2009, mAbs 1[6]:1-9; Holliger & Hudson, 2005, Nature Biotechnology 23[9]:1126-1136; expressly incorporated herein by reference). While these formats can be expressed at high levels in bacteria and may have favorable penetration benefits due to their small size, they dear rapidly in vivo and can present manufacturing obstacles related to their production and stability. A principal cause of these drawbacks is that antibody fragments typically lack the constant region of the antibody with its associated functional properties, including larger size, high stability, and binding to various Fc receptors and ligands that maintain long half-life in serum (i.e. the neonatal Fc receptor FcRn) or serve as binding sites for purification (i.e. protein A and protein G).

More recent work has attempted to address the shortcomings of fragment-based bispecifics by engineering dual binding into full length antibody -like formats (Wu et al., 2007, Nature Biotechnology 25[11]: 1290-1297; U.S. Ser. No. 12/477,711; Michaelson et al., 2009, mAbs 1[2]: 128-141; PCT/US2008/074693; Zuo et al., 2000, Protein Engineering 13[5]:361-367; U.S. Ser. No. 09/865,198; Shen et al., 2006, J Biol Chem 281[16]:10706-10714; Lu et al., 2005, J Biol Chem 280[20]:19665-19672; PCT/US2005/025472; expressly incorporated herein by reference). These formats overcome some of the obstacles of the antibody fragment bispecifics, principally because they contain an Fc region. One significant drawback of these formats is that, because they build new antigen binding sites on top of the homodimeric constant chains, binding to the new antigen is always bivalent.

For many antigens that are attractive as co-targets in a therapeutic bispecific format, the desired binding is monovalent rather than bivalent. For many immune receptors, cellular activation is accomplished by cross-linking of a monovalent binding interaction. The mechanism of cross-linking is typically mediated by antibody/antigen immune complexes, or via effector cell to target cell engagement. For example, the low affinity Fc gamma receptors (FcγRs) such as FcγRIIa, FcγRIIb, and FcγRIIIa bind monovalently to the antibody Fc region. Monovalent binding does not activate cells expressing these FcγRs; however, upon immune complexation or cell-to-cell contact, receptors are cross-linked and clustered on the cell surface, leading to activation. For receptors responsible for mediating cellular killing, for example FcγRIIIa on natural killer (NK) cells, receptor cross-linking and cellular activation occurs when the effector cell engages the target cell in a highly avid format (Bowles & Weiner, 2005, J Immunol Methods 304:88-99, expressly incorporated by reference). Similarly, on B cells the inhibitory receptor FcγRIIb downregulates B cell activation only when it engages into an immune complex with the cell surface B-cell receptor (BCR), a mechanism that is mediated by immune complexation of soluble IgG's with the same antigen that is recognized by the BCR (Heyman 2003, Immunol Lett 88[2]:157-161; Smith and Clatworthy, 2010, Nature Reviews Immunology 10:328-343; expressly incorporated by reference). As another example, CD3 activation of T-cells occurs only when its associated T-cell receptor (TCR) engages antigen-loaded MHC on antigen presenting cells in a highly avid cell-to-cell synapse (Kuhns et al., 2006, Immunity 24:133-139). Indeed nonspecific bivalent cross-linking of CD3 using an anti-CD3 antibody elicits a cytokine storm and toxicity (Perruche et al., 2009, J Immunol 183[2]:953-61; Chatenoud & Bluestone, 2007, Nature Reviews Immunology 7:622-632; expressly incorporated by reference). Thus for practical clinical use, the preferred mode of CD3 co-engagement for redirected killing of targets cells is monovalent binding that results in activation only upon engagement with the co-engaged target.

Thus while bispecifics generated from antibody fragments suffer biophysical and pharmacokinetic hurdles, a drawback of those built with full length antibody -like formats is that they engage co-target antigens multivalently in the absence of the primary target antigen, leading to nonspecific activation and potentially toxicity. The present invention solves this problem by introducing novel bispecific antibodies directed to CD3 and prostate specific membrane antigen (PSMA).

BRIEF SUMMARY

Provided herein are heterodimeric antibodies that bind to CD3 and prostrate specific membrane antigen (PSMA), a transmembrane protein that is considerably overexpressed on most prostate cancer cells. Such antibodies are useful for the treatment of cancers (e.g., prostrate cancer), for example, by facilitating the targeting CD3+T cells to PSMA expressing tumor cells.

In one aspect, provided herein is a heterodimeric antibody that includes: a) a first monomer that includes SEQ ID NO: 64; b) a second monomer that includes SEQ ID NO: 65; and c) a light chain comprising SEQ ID NO: 66. In a further aspect, provided herein is a nucleic acid composition that includes: a) a first nucleic acid encoding a first monomer that includes SEQ ID NO: 64; b) a second nucleic acid encoding a second monomer that includes SEQ ID NO: 65; and c) a third nucleic acid encoding a light chain that includes SEQ ID NO: 66. In another aspect, provided herein is an expression vector composition that includes: a) a first expression vector that includes a first nucleic acid encoding a first monomer that includes SEQ ID NO: 64; b) a second expression vector that includes a second nucleic acid encoding a second monomer that includes SEQ ID NO: 65; and c) a third expression vector that includes a third nucleic acid encoding a light chain that includes SEQ ID NO: 66.

In another aspect, provided herein is a heterodimeric antibody that includes: a) a first monomer that includes SEQ ID NO: 118; b) a second monomer that includes SEQ ID NO: 119; and c) a light chain that includes SEQ ID NO: 120. In a further aspect, provided herein is a nucleic acid composition that includes: a) a first nucleic acid encoding a first monomer that includes SEQ ID NO: 118; b) a second nucleic acid encoding a second monomer that includes SEQ ID NO: 119; and c) a third nucleic acid encoding a light chain that includes SEQ ID NO: 120. In another aspect, provided herein is an expression vector composition that includes: a) a first expression vector that includes a first nucleic acid encoding a first monomer that includes SEQ ID NO: 118; b) a second expression vector that includes a second nucleic acid encoding a second monomer that includes SEQ ID NO: 119; and c) a third expression vector comprising a nucleic acid that includes a third nucleic acid encoding a light chain that includes g SEQ ID NO: 120.

In one aspect, provided herein is a heterodimeric antibody that includes: a) a first monomer that includes SEQ ID NO: 121; b) a second monomer that includes SEQ ID NO: 122; and c) a light chain that includes SEQ ID NO: 123. In another aspect, provided herein is a nucleic acid composition that includes: a) a first nucleic acid encoding a first monomer that includes SEQ ID NO: 121; b) a second nucleic acid encoding a second monomer that includes SEQ ID NO: 122; and c) a third nucleic acid encoding a light chain that includes SEQ ID NO: 123. In a further aspect, provided herein is an expression vector composition that includes: a) a first expression vector that includes a first nucleic acid encoding a first monomer that includes SEQ ID NO: 121; b) a second expression vector that includes a second nucleic acid encoding a second monomer that includes SEQ ID NO: 122; and c) a third expression vector that includes a nucleic acid encoding a third nucleic acid encoding a light chain that includes SEQ ID NO: 123.

In one aspect, provided herein is a heterodimeric antibody that includes: a) a first monomer that includes SEQ ID NO: 124; b) a second monomer that includes SEQ ID NO: 125; and c) a light chain that includes SEQ ID NO: 126. In another aspect, provided herein is a nucleic acid composition that includes: a) a first nucleic acid encoding a first monomer that includes SEQ ID NO: 124; b) a second nucleic acid encoding a second monomer that includes SEQ ID NO: 125; and c) a third nucleic acid encoding a light chain that includes SEQ ID NO: 126. In yet another aspect, provided herein is an expression vector composition that includes: a) a first expression vector that includes a first nucleic acid encoding a first monomer that includes SEQ ID NO: 124; b) a second expression vector that includes a second nucleic acid encoding a second monomer that includes SEQ ID NO: 125; and c) a third expression vector that includes a nucleic acid encoding a third nucleic acid encoding a light chain that includes SEQ ID NO: 126.

In one aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a light chain. The first monomer includes: i) a first Fc domain; ii) an anti-CD3 scFv that includes a scFv variable light domain, and an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached to the N-terminus of the Fc domain using a domain linker. The second monomer includes a heavy chain that includes: i) a heavy variable domain; and ii) a heavy constant domain that includes a second Fc domain. The light chain includes a variable light domain and a constant light domain. The heavy variable domain and the variable light domain bind PSMA.

In another aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a light chain. The first monomer includes: i) a first Fc domain; and ii) an anti-CD3 scFv that includes a scFv variable light domain, an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached to the N-terminus of the Fc domain using a domain linker. The second monomer includes a heavy chain that includes i) a heavy variable domain; and ii) a heavy constant domain that includes a second Fc domain. The light chain includes a variable light domain and a constant light domain. The scFv variable light domain includes: the vlCDRs of CD3 L1.47 (a vlCDR1 having SEQ ID NO: 6, a vlCDR2 having SEQ ID NO: 7, and a vlCDR3 having SEQ ID NO: 8), the scFv variable heavy domain includes the vhCDRs of CD3 H1.30 (a vhCDR1 having SEQ ID NO: 2, a vhCDR2 having SEQ ID NO: 3, and a vhCDR3 having SEQ ID NO: 4), and the heavy variable domain and the variable light domain bind PSMA.

In another aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a light chain. The first monomer includes: i) a first Fc domain; and ii) an anti-CD3 scFv that includes a scFv variable light domain, an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached to the N-terminus of the Fc domain using a domain linker;. The second monomer includes a heavy chain that includes: i) a heavy variable domain; and ii) a heavy constant domain that includes a second Fc domain. The light chain includes a variable light domain and a constant light domain. The scFv variable light domain includes: the vlCDRs of CD3 L1.47 (a vlCDR1 having SEQ ID NO: 15, a vlCDR2 having SEQ ID NO: 16, and a vlCDR3 having SEQ ID NO: 17), the scFv variable heavy domain includes the vhCDRs of CD3 H1.32 (a vhCDR1 having SEQ ID NO: 11, a vhCDR2 having SEQ ID NO: 12, and a vhCDR3 having SEQ ID NO: 13), and the heavy variable domain and the variable light domain bind PSMA.

In one aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a light chain. The first monomer includes: i) a first Fc domain; and ii) an anti-CD3 scFv that includes a scFv variable light domain, an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached to the N-terminus of the Fc domain using a domain linker. The second monomer includes a heavy chain that includes: i) a heavy variable domain; and ii) a heavy constant domain that includes a second Fc domain. The light chain includes a variable light domain and a constant light domain. The scFv variable light domain includes the vlCDRs of CD3 L1.47 (a vlCDR1 having SEQ ID NO: 24, a vlCDR2 having SEQ ID NO: 25, and a vlCDR3 having SEQ ID NO: 26), the scFv variable heavy domain includes the vhCDRs of CD3 H1.89 (a vhCDR1 having SEQ ID NO: 20, a vhCDR2 having SEQ ID NO: 21, and a vhCDR3 having SEQ ID NO: 22), and the heavy variable domain and the variable light domain bind PSMA.

In one aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a light chain. The first monomer includes: i) a first Fc domain; and ii) an anti-CD3 scFv that includes a scFv variable light domain, an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached to the N-terminus of the Fc domain using a domain linker. The second monomer includes a heavy chain that includes: i) a heavy variable domain; and ii) a heavy constant domain that includes a second Fc domain. The light chain includes a variable light domain and a constant light domain. The scFv variable light domain includes: the vlCDRs of CD3 L1.47 (a vlCDR1 having SEQ ID NO: 33, a vlCDR2 having SEQ ID NO: 34, and a vlCDR3 having SEQ ID NO: 35), the scFv variable heavy domain includes the vhCDRs of CD3 H1.90 (a vhCDR1 having SEQ ID NO: 29, a vhCDR2 having SEQ ID NO: 30, and a vhCDR3 having SEQ ID NO: 31), and the heavy variable domain and the variable light domain bind PSMA.

In another aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a light chain. The first monomer includes: i) a first Fc domain; and ii) an anti-CD3 scFv that includes a scFv variable light domain, an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached to the N-terminus of the Fc domain using a domain linker. The second monomer includes a heavy chain that includes: i) a heavy variable domain; and ii) a heavy constant domain that includes a second Fc domain. The light chain includes a variable light domain and a constant light domain. The scFv variable light domain includes: the vlCDRs of CD3 L1.47 (a vlCDR1 having SEQ ID NO: 42, a vlCDR2 having SEQ ID NO: 43, and a vlCDR3 having SEQ ID NO: 44), the scFv variable heavy domain includes the vhCDRs of CD3 H1.33 (a vhCDR1 having SEQ ID NO: 38, a vhCDR2 having SEQ ID NO: 39, and a vhCDR3 having SEQ ID NO: 40), and the heavy variable domain and the variable light domain bind PSMA.

In another aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a light chain. The first monomer includes: i) a first Fc domain; and ii) an anti-CD3 scFv that includes a scFv variable light domain, an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached to the N-terminus of the Fc domain using a domain linker. The second monomer includes a heavy chain that includes: i) a heavy variable domain; and ii) a heavy constant domain that includes a second Fc domain. The light chain includes a variable light domain and a constant light domain. The scFv variable light domain includes: the vlCDRs of CD3 L1.47 (a vlCDR1 having SEQ ID NO: 51, a vlCDR2 having SEQ ID NO: 52, and a vlCDR3 having SEQ ID NO: 53), the scFv variable heavy domain includes the vhCDRs of CD3 H1.31 (a vhCDR1 having SEQ ID NO: 47, a vhCDR2 having SEQ ID NO: 48, and a vhCDR3 having SEQ ID NO: 49), and the heavy variable domain and the variable light domain bind PSMA.

In one embodiment of the antibodies described above, the heavy variable domain includes the vhCDRs of anti-PSMA J591 H1_L1 (a vhCDR1 having SEQ ID NO: 111, a vhCDR2 having SEQ ID NO: 112, and a vhCDR3 having SEQ ID NO: 113) and the variable light domain includes the vlCDRs of anti-PSMA J591 H1_L1 (a vlCDR1 having SEQ ID NO: 107, a vlCDR2 having SEQ ID NO: 108, and a vlCDR3 having SEQ ID NO: 109). In an exemplary embodiment, the heavy variable domain includes the variable heavy domain of anti-PSMA J591 H1_L1 (SEQ ID NO: 110) and the variable light domain includes the variable light domain of anti-PSMA J591 H1_L1 (SEQ ID NO: 106).

In one aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a light chain. The first monomer includes: i) a first Fc domain; and ii) an anti-CD3 scFv that includes a scFv variable light domain, an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached to the N-terminus of the Fc domain using a domain linker. The second monomer includes a heavy chain that includes: i) a heavy variable domain; and ii) a heavy constant domain that includes a second Fc domain. The light chain includes a variable light domain and a constant light domain. The heavy variable domain includes the vhCDRs of anti-PSMA J591 H1_L1 (a vhCDR1 having SEQ ID NO: 111, a vhCDR2 having SEQ ID NO: 112, and a vhCDR3 having SEQ ID NO: 113) and the variable light domain includes the vlCDRs of anti-PSMA J591 H1_L1 (a vlCDR1 having SEQ ID NO: 107, a vlCDR2 having SEQ ID NO: 108, and a vlCDR3 having SEQ ID NO: 109). The heavy variable domain and the variable light domain bind PSMA. In some embodiments, the heavy variable domain includes the variable heavy domain of anti-PSMA J591 H1_L1 (SEQ ID NO: 110) and the variable light domain includes the variable light domain of anti-PSMA J591 H1_L1 (SEQ ID NO: 106).

In some embodiments of the antibodies described above, the first Fc domain and the second Fc domain comprise a set of variants selected from the group consisting of S364K/E357Q: L368D/K370S; L368D/K370S: S364K; L368E/K370S: S364K; T411T/E360E/Q362E: D401K; L368D/K370S: S364K/E357L and K370S: S364K/E357Q.

In some embodiments of the antibodies described above, the scFv linker is a charged linker.

In some embodiments of the antibodies described above, the heavy chain constant domain includes the amino acid substitutions N208D/Q295E/N384D/Q418E/N421D.

In some embodiments of the antibodies described above, the first and second Fc domains include the amino acid substitutions E233P/L234V/L235A/G236del/S267K.

In another aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a common light chain. The first monomer includes: i) a first heavy chain that includes: 1) a first variable heavy domain; 2) a first constant heavy chain that includes a first Fc domain; and 3) a scFv that includes a scFv variable light domain, an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached to the C-terminus of the Fc domain using a domain linker. The second monomer includes a second heavy chain that includes a second variable heavy domain and a second constant heavy chain that includes a second Fc domain. The common light chain includes a variable light domain and a constant light domain. The first and the second Fc domains have a set of amino acid substitutions selected from the group consisting of S364K/E357Q: L368D/K370S; L368D/K370S: S364K; L368E/K370S: S364K; T411T/E360E/Q362E: D401K; L368D/K370S: S364K/E357L and K370S: S364K/E357Q. The first variable heavy domain and the variable light domain bind human PSMA (SEQ ID NO:131), the second variable heavy domain and the variable light domain bind human PSMA, and the scFv binds human CD3.

In one aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a common light chain. The first monomer includes i) a first heavy chain that includes: 1) a first variable heavy domain; 2) a first constant heavy domain that includes a first Fc domain; and 3) a first variable light domain, where the first variable light domain is covalently attached to the C-terminus of the first Fc domain using a domain linker. The second monomer includes: i) a second variable heavy domain; ii) a second constant heavy domain that includes a second Fc domain; and iii) a third variable heavy domain, where the third variable heavy domain is covalently attached to the C-terminus of the second Fc domain using a domain linker. The common light chain includes a second variable light domain and a constant light domain. The first and the second Fc domains have a set of amino acid substitutions selected from the group consisting of S364K/E357Q: L368D/K370S; L368D/K370S: S364K; L368E/K370S: S364K; T411T/E360E/Q362E: D401K; L368D/K370S: S364K/E357L and K370S: S364K/E357Q. The first variable heavy domain and the second variable light domain bind PSMA, the second variable heavy domain and the second variable light domain bind the PSMA, and the first variable light domain and the third variable heavy domain bind a CD3.

In one aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a common light chain. The first monomer includes i) a first heavy chain that includes i) a first heavy chain that includes:1) a first variable heavy domain; 2) a first constant heavy chain that includes a first CH1 domain and a first Fc domain; and 3) a scFv that includes a scFv variable light domain, an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached between the C-terminus of the CH1 domain and the N-terminus of the first Fc domain using domain linkers The second monomer includes a second heavy chain that includes a second variable heavy domain and a second constant heavy chain that includes a second Fc domain. The common light chain includes a variable light domain and a constant light domain. The first and the second Fc domain have a set of amino acid substitutions selected from the group consisting of S364K/E357Q: L368D/K370S; L368D/K370S: S364K; L368E/K370S: S364K; T411T/E360E/Q362E: D401K; L368D/K370S: S364K/E357L and K370S: S364K/E357Q. The first variable heavy domain and the variable light domain bind PSMA, the second variable heavy domain and the variable light domain bind PSMA, and the scFv binds CD3.

In one aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a common light chain. The first monomer includes i) a first heavy chain that includes i) a first variable heavy domain; ii) a first constant heavy domain that includes a first Fc domain; and iii) a first variable light domain, where the first variable light domain is covalently attached between the C-terminus of the CH1 domain of the first constant heavy domain and the N-terminus of the first Fc domain using domain linkers. The second monomer includes: i) a second variable heavy domain; ii) a second constant heavy domain that includes a second Fc domain; and iii) a third variable heavy domain, where the third variable heavy domain is covalently attached between the C-terminus of the CH1 domain of the second constant heavy domain and the N-terminus of the second Fc domain using domain linker. The common light chain includes a second variable light domain and a constant light domain. The first and the second Fc domains have a set of amino acid substitutions selected from the group consisting of S364K/E357Q: L368D/K370S; L368D/K370S: S364K; L368E/K370S: S364K; T411T/E360E/Q362E: D401K; L368D/K370S: S364K/E357L and K370S: S364K/E357Q. The first variable heavy domain and the second variable light domain bind PSMA, the second variable heavy domain and the second variable light domain bind PSMA, and the first variable light domain and the third variable heavy domain binds CD3.

In one aspect, provided herein is a heterodimeric antibody that includes a first monomer, a second monomer and a light chain. The first monomer includes i) a first heavy chain that includes i) a first heavy chain that includes: 1) a first variable heavy domain; 2) a first constant heavy chain that includes a first CH1 domain and a first Fc domain; and 3) a scFv that includes a scFv variable light domain, an scFv linker and a scFv variable heavy domain; where the scFv is covalently attached between the C-terminus of the CH1 domain and the N-terminus of the first Fc domain using domain linkers The second monomer includes a second Fc domain. The light chain includes a variable light domain and a constant light domain. The first and the second Fc domain have a set of amino acid substitutions selected from the group consisting of S364K/E357Q: L368D/K370S; L368D/K370S: S364K; L368E/K370S: S364K; T411T/E360E/Q362E: D401K; L368D/K370S: S364K/E357L and K370S: S364K/E357Q. The first variable heavy domain and the variable light domain bind a first antigen, the scFv binds a second antigen, and one of the first and second antigens is PSMA and the other is CD3.

In another aspect, provided herein is a nucleic acid composition that encodes any of the subject heterodimeric antibodies described herein. In some embodiments, the nucleic acid composition includes a) a first nucleic acid encoding the first monomer; b) a second nucleic acid encoding the second monomer; and c) a third nucleic acid encoding the light chain.

In another aspect, provided herein is a heterodimeric antibody selected from the group consisting of: XENP14484, XENP16873, XENP16874 and XENP19722.

In another aspect, provided herein is an expression vector composition that includes any one of the nucleic acid compositions described herein. In another aspect, provided herein is a host cell that includes any one of the nucleic acid compositions or expression vector compositions described herein. In yet another aspect, provided herein is a method of making a subject heterodimeric antibody described herein that includes culturing a host cell described herein under conditions where the antibody is expressed, and recovering the antibody.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A and 1B depict several formats of the present invention. Two forms of the “bottle opener” format are depicted, one with the anti-CD3 antigen binding domain comprising a scFv and the anti-PSMA antigen binding domain comprising a Fab, and one with these reversed. The mAb-Fv, mAb-scFv, Central-scFv and Central-Fv, common light chain, and bispecific IgG formats are all shown. While each of the structures in these figures depict the anti-PSMA and anti-CD3 domains as an scFv or a Fv, the anti-PSMA and anti-CD3 domains of each of these structures can also be “reversed”. For example, it is also contemplated that the mAb-scFv depicted in FIG. 1A can have an anti-PSMA scFv and an anti-CD3 Fv. In addition, “one-armed” formats, where one monomer just comprises an Fc domain, are shown, including a one arm Central-scFv and a one arm Central-Fv. A dual scFv format is also shown.

FIG. 2 depicts the sequences of the “High CD3” anti-CD3_H1.30_L1.47 construct, including the variable heavy and light domains (CDRs underlined), as well as the individual vl and vhCDRs, as well as an scFv construct with a charged linker (double underlined). As is true of all the sequences depicted in the Figures, this charged linker may be replaced by an uncharged linker or a different charged linker, as needed.

FIG. 3 depicts the sequences of the “High-Int #1” Anti-CD3_H1.32_L1.47 construct, including the variable heavy and light domains (CDRs underlined), as well as the individual vl and vhCDRs, as well as an scFv construct with a charged linker (double underlined). As is true of all the sequences depicted in the Figures, this charged linker may be replaced by an uncharged linker or a different charged linker, as needed.

FIG. 4 depicts the sequences of the “High-Int #2” Anti-CD3_H1.89_L1.47 construct, including the variable heavy and light domains (CDRs underlined), as well as the individual vl and vhCDRs, as well as an scFv construct with a charged linker (double underlined). As is true of all the sequences depicted in the Figures, this charged linker may be replaced by an uncharged linker or a different charged linker, as needed.

FIG. 5 depicts the sequences of the “High-Int #3” Anti-CD3_H1.90_L1.47 construct, including the variable heavy and light domains (CDRs underlined), as well as the individual vl and vhCDRs, as well as an scFv construct with a charged linker (double underlined). As is true of all the sequences depicted in the Figures, this charged linker may be replaced by an uncharged linker or a different charged linker, as needed.

FIG. 6 depicts the sequences of the “Int” Anti-CD3_H1.33_L1.47 construct, including the variable heavy and light domains (CDRs underlined), as well as the individual vl and vhCDRs, as well as an scFv construct with a charged linker (double underlined). As is true of all the sequences depicted in the Figures, this charged linker may be replaced by an uncharged linker or a different charged linker (e.g., any charged linker in FIG. 14), as needed.

FIG. 7 depicts the sequences of the “Low” Anti-CD3_H1.31_L1.47 construct, including the variable heavy and light domains (CDRs underlined), as well as the individual vl and vhCDRs, as well as an scFv construct with a charged linker (double underlined). As is true of all the sequences depicted in the Figures, this charged linker may be replaced by an uncharged linker or a different charged linker (e.g., any charged linker in FIG. 14), as needed.

FIG. 8 depicts the sequences of the variable heavy and variable light domains of XENP14484, including the variable heavy and light domains (CDRs underlined), as well as the individual vl and vhCDRs, as well as an scFv construct with a charged linker (double underlined). However, as will be appreciated by those in the art, the charged linker in the case of the PSMA embodiment, the positively charged linker can also be replaced by a negatively charged one as shown in FIG. 14, or an uncharged traditional GS linker.

FIG. 9 depicts the sequences of the XENP14484.

FIGS. 10A-10E depict useful pairs of heterodimerization variant sets (including skew and pI variants). On FIG. 10E, there are variants for which there are no corresponding “monomer 2” variants; these are pI variants which can be used alone on either monomer, or included on the Fab side of a bottle opener, for example, and an appropriate charged scFv linker can be used on the second monomer that utilizes a scFv as the second antigen binding domain. Suitable charged linkers are shown in FIG. 14.

FIG. 11 depict a list of isosteric variant antibody constant regions and their respective substitutions. pI_(−) indicates lower pI variants, while pI_(+) indicates higher pI variants. These can be optionally and independently combined with other heterodimerization variants of the invention (and other variant types as well, as outlined herein).

FIG. 12 depict useful ablation variants that ablate FcγR binding (sometimes referred to as “knock outs” or “KO” variants).

FIG. 13 show two particularly useful embodiments of the invention.

FIGS. 14A-14B depict a number of charged scFv linkers that find use in increasing or decreasing the pI of heterodimeric antibodies that utilize one or more scFv as a component. The (+H) positive linker finds particular use herein, particularly with anti-CD3 vl and vh sequences shown herein. A single prior art scFv linker with a single charge is referenced as “Whitlow”, from Whitlow et al., Protein Engineering 6(8):989-995 (1993). It should be noted that this linker was used for reducing aggregation and enhancing proteolytic stability in scFvs.

FIG. 15 depicts a list of engineered heterodimer-skewing Fc variants with heterodimer yields (determined by HPLC-CIEX) and thermal stabilities (determined by DSC). Not determined thermal stability is denoted by “n.d.”.

FIG. 16. Test articles are anti-PSMA or anti-RSV x anti-CD3 bispecifics. Top panel: Cell surface binding to 100k LNCaP cells. Detection was by a FITC-labeled anti-human IgG Fc secondary antibody. Bottom panel: Redirected T cell cytotoxicity assay, 24 h incubation, 10 k LNCaP cells, 400 k purified T cells. Detection was by LDH.

FIGS. 17A and 17B include graphs showing that T cell response and cytokine release syndrome (CRS) is dependent on the engagement of target cells in monkeys. Cynomolgus monkeys (n=3) were given a single dose of either XENP14484 (anti-PSMA×anti-CD3, 30 μg/kg) or XENP13245 (anti-RSV×CD3, 3 mg/kg). FIG. 17A CD4 T cell margination (top panel) and activation by CD69 MFI (middle panel) were examined by flow cytometry. IL-6 serum levels were examined by ELISA (bottom panel). FIG. 17B CD8⁺ T cell margination (top panel) and activation by CD69 MFI (middle panel) were examined by flow cytometry. TNF serum levels were examined by ELISA (bottom panel).

FIG. 18 shows a matrix of possible combinations for embodiments of the invention. An “A” means that the CDRs of the referenced anti-CD3 sequences can be combined with the CDRs of the anti-PSMA construct on the left hand side. That is, for example for the top left hand cell, the vhCDRs from the variable heavy chain anti-CD3 H1.30 sequence and the vlCDRs from the variable light chain of anti-CD3 L1.47 sequence can be combined with the vhCDRs from the anti-PSMA J591 H1_L1 sequence and the vlCDRs from the J591 H1_L1 sequence (see FIG. 20). A “B” means that the CDRs from the anti-CD3 constructs can be combined with the variable heavy and light domains from the anti-PSMA construct. That is, for example for the top left hand cell, the vhCDRs from the variable heavy chain anti-CD3 H1.30 sequence and the vlCDRs from the variable light chain of anti-CD3 L1.47 sequence can be combined with the variable heavy domain J591 H1_L1 sequence and the J591 H1_L1 sequence. A “C” is reversed, such that the variable heavy domain and variable light domain from the CD3 sequences are used with the CDRs of the PSMA sequences. A “D” is where both the variable heavy and variable light chains from each are combined. An “E” is where the scFv of the anti-CD3 is used with the CDRs of the PSMA antigen binding domain construct. An “F” is where the scFv of the CD3 is used with the variable heavy and variable light domains of the PSMA antigen binding domain. A “G” is where the CDRs of the anti-CD3 constructs are used with the scFv of the PSMA constuct. An “H” is where the variable heavy domain and variable light domain of the anti-CD3 is used with the scFv of the anti-PSMA.

FIG. 19 depicts amino acid sequences for various prostate-specific membrane antigens (PSMAs), including full human PSMA, human PSMA extracellular domain, full Macaca fascicularis PSMA, and Macaca fascicularis PSMA extracellular domain.

FIG. 20 depicts the amino acid sequences for the variable heavy (VH) and variable light (VL) domains of unhumanized anti-PSMA antibody J591 and humanized anti-PSMA antibody J591 H1_L1. Also depicted are the complementary determining regions of the variable heavy (vhCDR1-3) and variable light (vlCDR1-3) domains included in these variable heavy and variable light domains. In some embodiments, the anti-PSMA CDRs and VH and VLs can be used in monospecific and heterodimer antibodies (e.g., anti-PSMA×anti-CD3) antibodies described herein. In some embodiments, wherein the heterodimeric antibody further includes an anti-CD3 binding domain, the anti-CD3 binding domain can include any of the VH, VL or CDRs depicted in FIGS. 5-7.

FIGS. 21A-21E depict the amino acid sequences of exemplary anti-PSMA x anti-CD3 antibodies described herein, including XENP14484 (FIG. 21A), XENP16873 (FIG. 21B), XENP16874 (FIG. 21C), and XENP19722 (FIG. 21D). FIG. 22E depicts the amino acid sequence of an exemplary bottle opener scaffold. Such a scaffold can be combined with the various variable heavy domains, variable light domains, and scFvs described herein.

FIG. 22 depicts a graph showing the PSMA affinity (Kd) determination of anti-PSMA×anti-CD3 antibodies XENP14484 and XENP19722.

FIG. 23 are graphs showing the binding of exemplary anti-PSMA x anti-CD3 antibodies XENP14484 and XENP 19722 to LNCaP prostate cancer cells (LNCaP)(top) and the ability of such exemplary antibodies to mediate redirected T cell cytotoxicity (RTCC) of a LNCaP prostate cancer cell line (bottom). Top panel: Cell surface binding to 100 k LNCaP cells. Detection was by a FITC-labeled anti-human IgG Fc secondary antibody. Bottom panel: Redirected T cell cytotoxicity assay, overnight incubation, 10 k LNCaP cells, 100 k purified T cells from human PBMC. Detection was by LDH.

FIG. 24 are graphs showing that an anti-PSMA×anti-CD3 bispecific antibody with reduced CD3 affinity (XENP19722) exhibits similar T cell activation (top, middle) but lower CRS in monkeys as compared to an anti-PSMA x anti-CD3 bispecific antibody with high CD3 affinity (XENP14484)(bottom).

FIGS. 25A-25D shows the sequences of several useful bottle opener format backbones, without the Fv sequences (e.g. the scFv and the vh and vl for the Fab side). As will be appreciated by those in the art and outlined below, these sequences can be used with any vh and vl pairs outlined herein, with one monomer including a scFv (optionally including a charged scFv linker) and the other monomer including the Fab sequences (e.g. a vh attached to the “Fab side heavy chain” and a vl attached to the “constant light chain”). The scFv can be anti-CD3 or anti-TTA, with the Fab being the other. That is, any anti-CD3 and anti-PSMA Fv sequences outlined herein, including the CDRs of such sequences, for can be incorporated into these FIG. 25 backbones in any combination.

It should be noted that these bottle opener backbones find use in the Central-scFv format of FIG. 1B, where an additional, second Fab (vh-CH1 and vl-constant light) with the same antigen binding as the first Fab is added to the N-terminus of the scFv on the “bottle opener side”.

DETAILED DESCRIPTION OF THE INVENTION I. Definitions

In order that the application may be more completely understood, several definitions are set forth below. Such definitions are meant to encompass grammatical equivalents.

By “ablation” herein is meant a decrease or removal of activity. Thus for example, “ablating FcγR binding” means the Fc region amino acid variant has less than 50% starting binding as compared to an Fc region not containing the specific variant, with less than 70-80-90-95-98% loss of activity being preferred, and in general, with the activity being below the level of detectable binding in a Biacore assay. Of particular use in the ablation of FcγR binding are those shown in FIG. 12.

By “ADCC” or “antibody dependent cell-mediated cytotoxicity” as used herein is meant the cell-mediated reaction wherein nonspecific cytotoxic cells that express FcγRs recognize bound antibody on a target cell and subsequently cause lysis of the target cell. ADCC is correlated with binding to FcγRIIIa; increased binding to FcγRIIIa leads to an increase in ADCC activity.

By “ADCP” or antibody dependent cell-mediated phagocytosis as used herein is meant the cell-mediated reaction wherein nonspecific cytotoxic cells that express FcγRs recognize bound antibody on a target cell and subsequently cause phagocytosis of the target cell.

By “modification” herein is meant an amino acid substitution, insertion, and/or deletion in a polypeptide sequence or an alteration to a moiety chemically linked to a protein. For example, a modification may be an altered carbohydrate or PEG structure attached to a protein. By “amino acid modification” herein is meant an amino acid substitution, insertion, and/or deletion in a polypeptide sequence. For clarity, unless otherwise noted, the amino acid modification is always to an amino acid coded for by DNA, e.g. the 20 amino acids that have codons in DNA and RNA.

By “amino acid substitution” or “substitution” herein is meant the replacement of an amino acid at a particular position in a parent polypeptide sequence with a different amino acid. In particular, in some embodiments, the substitution is to an amino acid that is not naturally occurring at the particular position, either not naturally occurring within the organism or in any organism. For example, the substitution E272Y refers to a variant polypeptide, in this case an Fc variant, in which the glutamic acid at position 272 is replaced with tyrosine. For clarity, a protein which has been engineered to change the nucleic acid coding sequence but not change the starting amino acid (for example exchanging CGG (encoding arginine) to CGA (still encoding arginine) to increase host organism expression levels) is not an “amino acid substitution”; that is, despite the creation of a new gene encoding the same protein, if the protein has the same amino acid at the particular position that it started with, it is not an amino acid substitution.

By “amino acid insertion” or “insertion” as used herein is meant the addition of an amino acid sequence at a particular position in a parent polypeptide sequence. For example, -233E or 233E designates an insertion of glutamic acid after position 233 and before position 234. Additionally, -233ADE or A233ADE designates an insertion of AlaAspGlu after position 233 and before position 234.

By “amino acid deletion” or “deletion” as used herein is meant the removal of an amino acid sequence at a particular position in a parent polypeptide sequence. For example, E233- or E233# or E233()designates a deletion of glutamic acid at position 233. Additionally, EDA233- or EDA233# designates a deletion of the sequence GluAspAla that begins at position 233.

By “variant protein” or “protein variant”, or “variant” as used herein is meant a protein that differs from that of a parent protein by virtue of at least one amino acid modification. Protein variant may refer to the protein itself, a composition comprising the protein, or the amino sequence that encodes it. Preferably, the protein variant has at least one amino acid modification compared to the parent protein, e.g. from about one to about seventy amino acid modifications, and preferably from about one to about five amino acid modifications compared to the parent. As described below, in some embodiments the parent polypeptide, for example an Fc parent polypeptide, is a human wild type sequence, such as the Fc region from IgG1, IgG2, IgG3 or IgG4, although human sequences with variants can also serve as “parent polypeptides”, for example a IgG1/2 hybrid as previously disclosed. The protein variant sequence herein will preferably possess at least about 80% identity with a parent protein sequence, and most preferably at least about 90% identity, more preferably at least about 95-98-99% identity . Variant protein can refer to the variant protein itself, compositions comprising the protein variant, or the DNA sequence that encodes it. Accordingly, by “antibody variant” or “variant antibody” as used herein is meant an antibody that differs from a parent antibody by virtue of at least one amino acid modification, “IgG variant” or “variant IgG” as used herein is meant an antibody that differs from a parent IgG (again, in many cases, from a human IgG sequence) by virtue of at least one amino acid modification, and “immunoglobulin variant” or “variant immunoglobulin” as used herein is meant an immunoglobulin sequence that differs from that of a parent immunoglobulin sequence by virtue of at least one amino acid modification. “Fc variant” or “variant Fc” as used herein is meant a protein comprising an amino acid modification in an Fc domain. The Fc variants of the present invention are defined according to the amino acid modifications that compose them. Thus, for example, N434S or 434S is an Fc variant with the substitution serine at position 434 relative to the parent Fc polypeptide, wherein the numbering is according to the EU index. Likewise, M428L/N434S defines an Fc variant with the substitutions M428L and N434S relative to the parent Fc polypeptide. The identity of the WT amino acid may be unspecified, in which case the aforementioned variant is referred to as 428L/434S. It is noted that the order in which substitutions are provided is arbitrary, that is to say that, for example, 428L/434S is the same Fc variant as M428L/N434S, and so on. For all positions discussed in the present invention that relate to antibodies, unless otherwise noted, amino acid position numbering is according to the EU index. The EU index or EU index as in Kabat or EU numbering scheme refers to the numbering of the EU antibody (Edelman et al., 1969, Proc Natl Acad Sci USA 63:78-85, hereby entirely incorporated by reference.) The modification can be an addition, deletion, or substitution. Substitutions can include naturally occurring amino acids and, in some cases, synthetic amino acids. Examples include U.S. Pat. No. 6,586,207; WO 98/48032; WO 03/073238; US2004-0214988A1; WO 05/35727A2; WO 05/74524A2; J. W. Chin et al., (2002), Journal of the American Chemical Society 124:9026-9027; J. W. Chin, & P. G. Schultz, (2002), ChemBioChem 11:1135-1137; J. W. Chin, et al., (2002), PICAS United States of America 99:11020-11024; and, L. Wang, & P. G. Schultz, (2002), Chem. 1-10, all entirely incorporated by reference.

As used herein, “protein” herein is meant at least two covalently attached amino acids, which includes proteins, polypeptides, oligopeptides and peptides. The peptidyl group may comprise naturally occurring amino acids and peptide bonds, or synthetic peptidomimetic structures, i.e. “analogs”, such as peptoids (see Simon et al., PNAS USA 89(20):9367 (1992), entirely incorporated by reference). The amino acids may either be naturally occurring or synthetic (e.g. not an amino acid that is coded for by DNA); as will be appreciated by those in the art. For example, homo-phenylalanine, citrulline, ornithine and noreleucine are considered synthetic amino acids for the purposes of the invention, and both D- and L-(R or S) configured amino acids may be utilized. The variants of the present invention may comprise modifications that include the use of synthetic amino acids incorporated using, for example, the technologies developed by Schultz and colleagues, including but not limited to methods described by Cropp & Shultz, 2004, Trends Genet. 20(12):625-30, Anderson et al., 2004, Proc Natl Acad Sci USA 101 (2):7566-71, Zhang et al., 2003, 303(5656):371-3, and Chin et al., 2003, Science 301(5635):964-7, all entirely incorporated by reference. In addition, polypeptides may include synthetic derivatization of one or more side chains or termini, glycosylation, PEGylation, circular permutation, cyclization, linkers to other molecules, fusion to proteins or protein domains, and addition of peptide tags or labels.

By “residue” as used herein is meant a position in a protein and its associated amino acid identity. For example, Asparagine 297 (also referred to as Asn297 or N297) is a residue at position 297 in the human antibody IgG1.

By “Fab” or “Fab region” as used herein is meant the polypeptide that comprises the VH, CH1, VL, and CL immunoglobulin domains. Fab may refer to this region in isolation, or this region in the context of a full length antibody, antibody fragment or Fab fusion protein. By “Fv” or “Fv fragment” or “Fv region” as used herein is meant a polypeptide that comprises the VL and VH domains of a single antibody. As will be appreciated by those in the art, these generally are made up of two chains.

By “IgG subclass modification” or “isotype modification” as used herein is meant an amino acid modification that converts one amino acid of one IgG isotype to the corresponding amino acid in a different, aligned IgG isotype. For example, because IgG1 comprises a tyrosine and IgG2 a phenylalanine at EU position 296, a F296Y substitution in IgG2 is considered an IgG subclass modification.

By “non-naturally occurring modification” as used herein is meant an amino acid modification that is not isotypic. For example, because none of the IgGs comprise a serine at position 434, the substitution 434S in IgG1, IgG2, IgG3, or IgG4 (or hybrids thereof) is considered a non-naturally occurring modification.

By “amino acid” and “amino acid identity” as used herein is meant one of the 20 naturally occurring amino acids that are coded for by DNA and RNA.

By “effector function” as used herein is meant a biochemical event that results from the interaction of an antibody Fc region with an Fc receptor or ligand. Effector functions include but are not limited to ADCC, ADCP, and CDC.

By “IgG Fc ligand” as used herein is meant a molecule, preferably a polypeptide, from any organism that binds to the Fc region of an IgG antibody to form an Fc/Fc ligand complex. Fc ligands include but are not limited to FcγRIs, FcγRIIs, FcγRIIIs, FcRn, C1q, C3, mannan binding lectin, mannose receptor, staphylococcal protein A, streptococcal protein G, and viral FcγR. Fc ligands also include Fc receptor homologs (FcRH), which are a family of Fc receptors that are homologous to the FcγRs (Davis et al., 2002, Immunological Reviews 190:123-136, entirely incorporated by reference). Fc ligands may include undiscovered molecules that bind Fc. Particular IgG Fc ligands are FcRn and Fc gamma receptors. By “Fc ligand” as used herein is meant a molecule, preferably a polypeptide, from any organism that binds to the Fc region of an antibody to form an Fc/Fc ligand complex.

By “Fc gamma receptor”, “FcγR” or “FcqammaR” as used herein is meant any member of the family of proteins that bind the IgG antibody Fc region and is encoded by an FcγR gene. In humans this family includes but is not limited to FcγRI (CD64), including isoforms FcγRla, FcγRIb, and FcγRIc; FcγRII (CD32), including isoforms FcγRIIa (including allotypes H131 and R131), FcγRIIb (including FcγRIIb-1 and FcγRIIb-2), and FcγRIIc; and FcγRIII (CD16), including isoforms FcγRIIIa (including allotypes V158 and F158) and FcγRIIIb (including allotypes FcγRIIb-NA1 and FcγRIIb-NA2) (Jefferis et at, 2002, Immunol Lett 82:57-65, entirely incorporated by reference), as well as any undiscovered human FcγRs or FcγR isoforms or allotypes. An FcγR may be from any organism, including but not limited to humans, mice, rats, rabbits, and monkeys. Mouse FcγRs include but are not limited to FcγRI (CD64), FcγRII (CD32), FcγRIII (CD16), and FcγRIII-2 (CD16-2), as well as any undiscovered mouse FcγRs or FcγR isoforms or allotypes.

By “FcRn” or “neonatal Fc Receptor” as used herein is meant a protein that binds the IgG antibody Fc region and is encoded at least in part by an FcRn gene. The FcRn may be from any organism, including but not limited to humans, mice, rats, rabbits, and monkeys. As is known in the art, the functional FcRn protein comprises two polypeptides, often referred to as the heavy chain and light chain. The light chain is beta-2-microglobulin and the heavy chain is encoded by the FcRn gene. Unless otherwise noted herein, FcRn or an FcRn protein refers to the complex of FcRn heavy chain with beta-2-microglobulin. A variety of FcRn variants are used to increase binding to the FcRn receptor, and in some cases, to increase serum half-life as outlined herein, such as 428L/434S.

By “parent polypeptide” as used herein is meant a starting polypeptide that is subsequently modified to generate a variant. The parent polypeptide may be a naturally occurring polypeptide, or a variant or engineered version of a naturally occurring polypeptide. Parent polypeptide may refer to the polypeptide itself, compositions that comprise the parent polypeptide, or the amino acid sequence that encodes it. Accordingly, by “parent immunoglobulin” as used herein is meant an unmodified immunoglobulin polypeptide that is modified to generate a variant, and by “parent antibody” as used herein is meant an unmodified antibody that is modified to generate a variant antibody. It should be noted that “parent antibody” includes known commercial, recombinantly produced antibodies as outlined below.

By “Fc” or “Fc region” or “Fc domain” as used herein is meant the polypeptide comprising the constant region of an antibody excluding the first constant region immunoglobulin domain and in some cases, part of the hinge. Thus Fc refers to the last two constant region immunoglobulin domains of IgA, IgD, and IgG, the last three constant region immunoglobulin domains of IgE and IgM, and the flexible hinge N-terminal to these domains. For IgA and IgM, Fc may include the J chain. For IgG, the Fc domain comprises immunoglobulin domains Cγ2 and Cγ3 (Cγ2 and Cγ3) and the lower hinge region between Cγ1 (Cγ1) and Cγ2 (Cγ2). Although the boundaries of the Fc region may vary, the human IgG heavy chain Fc region is usually defined to include residues C226 or P230 to its carboxyl-terminus, wherein the numbering is according to the EU index as in Kabat. In some embodiments, as is more fully described below, amino acid modifications are made to the Fc region, for example to alter binding to one or more FcγR receptors or to the FcRn receptor.

By “heavy constant region” herein is meant the CH1-hinge-CH2-CH3 portion of an antibody.

By “Fc fusion protein” or “immunoadhesin” herein is meant a protein comprising an Fc region, generally linked (optionally through a linker moiety, as described herein) to a different protein, such as a binding moiety to a target protein, as described herein. In some cases, one monomer of the heterodimeric antibody comprises an antibody heavy chain (either including an scFv or further including a light chain) and the other monomer is a Fc fusion, comprising a variant Fc domain and a ligand. In some embodiments, these “half antibody-half fusion proteins” are referred to as “Fusionbodies”.

By “position” as used herein is meant a location in the sequence of a protein. Positions may be numbered sequentially, or according to an established format, for example the EU index for antibody numbering.

By “target antigen” as used herein is meant the molecule that is bound specifically by the variable region of a given antibody. A target antigen may be a protein, carbohydrate, lipid, or other chemical compound. A wide number of suitable target antigens are described below.

By “strandedness” in the context of the monomers of the heterodimeric antibodies of the invention herein is meant that, similar to the two strands of DNA that “match”, heterodimerization variants are incorporated into each monomer so as to preserve the ability to “match” to form heterodimers. For example, if some pI variants are engineered into monomer A (e.g. making the pI higher) then steric variants that are “charge pairs” that can be utilized as well do not interfere with the pI variants, e.g. the charge variants that make a pI higher are put on the same “strand” or “monomer” to preserve both functionalities. Similarly, for “skew” variants that come in pairs of a set as more fully outlined below, the skilled artisan will consider pI in deciding into which strand or monomer that incorporates one set of the pair will go, such that pI separation is maximized using the pI of the skews as well.

By “target cell” as used herein is meant a cell that expresses a target antigen.

By “variable region” as used herein is meant the region of an immunoglobulin that comprises one or more Ig domains substantially encoded by any of the V.kappa., V.lamda., and/or VH genes that make up the kappa, lambda, and heavy chain immunoglobulin genetic loci respectively.

By “wild type or WT” herein is meant an amino acid sequence or a nucleotide sequence that is found in nature, including allelic variations. A WT protein has an amino acid sequence or a nucleotide sequence that has not been intentionally modified.

The antibodies of the present invention are generally isolated or recombinant. “Isolated,” when used to describe the various polypeptides disclosed herein, means a polypeptide that has been identified and separated and/or recovered from a cell or cell culture from which it was expressed. Ordinarily, an isolated polypeptide will be prepared by at least one purification step. An “isolated antibody,” refers to an antibody which is substantially free of other antibodies having different antigenic specificities. “Recombinant” means the antibodies are generated using recombinant nucleic acid techniques in exogeneous host cells.

“Specific binding” or “specifically binds to” or is “specific for” a particular antigen or an epitope means binding that is measurably different from a non-specific interaction. Specific binding can be measured, for example, by determining binding of a molecule compared to binding of a control molecule, which generally is a molecule of similar structure that does not have binding activity. For example, specific binding can be determined by competition with a control molecule that is similar to the target.

Specific binding for a particular antigen or an epitope can be exhibited, for example, by an antibody having a KD for an antigen or epitope of at least about 10-4 M, at least about 10-5 M, at least about 10-6 M, at least about 10-7 M, at least about 10-8 M, at least about 10-9 M, alternatively at least about 10-10 M, at least about 10-11 M, at least about 10-12 M, or greater, where KD refers to a dissociation rate of a particular antibody-antigen interaction. Typically, an antibody that specifically binds an antigen will have a KD that is 20-, 50-, 100-, 500-, 1000-, 5,000-, 10,000- or more times greater for a control molecule relative to the antigen or epitope.

Also, specific binding for a particular antigen or an epitope can be exhibited, for example, by an antibody having a KA or Ka for an antigen or epitope of at least 20-, 50-, 100-, 500-,1000-, 5,000-, 10,000- or more times greater for the epitope relative to a control, where KA or Ka refers to an association rate of a particular antibody-antigen interaction.

II. Overview

Bispecific antibodies that co-engage CD3 and a tumor antigen target have been designed and used to redirect T cells to attack and lyse targeted tumor cells. Examples include the BiTE and DART formats, which monovalently engage CD3 and a tumor antigen. While the CD3-targeting approach has shown considerable promise, a common side effect of such therapies is the associated production of cytokines, often leading to toxic cytokine release syndrome. Because the anti-CD3 binding domain of the bispecific antibody engages all T cells, the high cytokine-producing CD4 T cell subset is recruited. Moreover, the CD4 T cell subset includes regulatory T cells, whose recruitment and expansion can potentially lead to immune suppression and have a negative impact on long-term tumor suppression. In addition, these formats do not contain Fc domains and show very short serum half-lives in patients.

While the CD3-targeting approach has shown considerable promise, a common side effect of such therapies is the associated production of cytokines, often leading to toxic cytokine release syndrome. Because the anti-CD3 binding domain of the bispecific antibody engages all T cells, the high cytokine-producing CD4 T cell subset is recruited. Moreover, the CD4 T cell subset includes regulatory T cells, whose recruitment and expansion can potentially lead to immune suppression and have a negative impact on long-term tumor suppression. One such possible way to reduce cytokine production and possibly reduce the activation of CD4 T cells is by reducing the affinity of the anti-CD3 domain for CD3.

Accordingly, in some embodiments the present invention provides antibody constructs comprising anti-CD3 antigen binding domains in combination with antigen binding domains that bind prostate specific membrane antigen (PSMA).

Furthermore, in some embodiments, the anti-CD3 antigen binding domains are “strong” or “high affinity” binders to CD3 (e.g. one example are heavy and light variable domains depicted as H1.30_L1.47 (optionally including a charged linker as appropriate)) and also bind to PSMA. In other embodiments, the present invention provides antibody constructs comprising anti-CD3 antigen binding domains that are “lite” or “lower affinity” binders to CD3. Additional embodiments provides antibody constructs comprising anti-CD3 antigen binding domains that have intermediate or “medium” affinity to CD3 that also bind to PSMA. Affinity is generally measured using a Biacore assay.

It should be appreciated that the “high, medium, low” anti-CD3 sequences of the present invention can be used in a variety of heterodimerization formats. While the majority of the disclosure herein uses the “bottle opener” format of heterodimers, these variable heavy and light sequences, as well as the scFv sequences (and Fab sequences comprising these variable heavy and light sequences) can be used in other formats, such as those depicted in FIG. 2 of WO Publication No. 2014/145806, the Figures, formats and legend of which is expressly incorporated herein by reference.

Accordingly, the present invention provides heterodimeric antibodies that bind to two different antigens, e.g the antibodies are “bispecific”, in that they bind two different target antigens, e.g. CD3 and PSMA in the present invention. These heterodimeric antibodies can bind these target antigens either monovalently (e.g. there is a single antigen binding domain such as a variable heavy and variable light domain pair) or bivalently (there are two antigen binding domains that each independently bind the antigen). The heterodimeric antibodies of the invention are based on the use different monomers which contain amino acid substitutions that “skew” formation of heterodimers over homodimers, as is more fully outlined below, coupled with “pI variants” that allow simple purification of the heterodimers away from the homodimers, as is similarly outlined below. For the heterodimeric bispecific antibodies of the invention, the present invention generally relies on the use of engineered or variant Fc domains that can self-assemble in production cells to produce heterodimeric proteins, and methods to generate and purify such heterodimeric proteins.

III. Antibodies

The present invention relates to the generation of bispecific antibodies that bind CD3 and PSMA, generally therapeutic antibodies. As is discussed below, the term “antibody” is used generally. Antibodies that find use in the present invention can take on a number of formats as described herein, including traditional antibodies as well as antibody derivatives, fragments and mimetics, described herein.

Traditional antibody structural units typically comprise a tetramer. Each tetramer is typically composed of two identical pairs of polypeptide chains, each pair having one “light” (typically having a molecular weight of about 25 kDa) and one “heavy” chain (typically having a molecular weight of about 50-70 kDa). Human light chains are classified as kappa and lambda light chains. The present invention is directed to the IgG class, which has several subclasses, including, but not limited to IgG1, IgG2, IgG3, and IgG4. It should be noted that IgG1 has different allotypes with polymorphisms at 356 (D or E) and 358 (L or M). The sequences depicted herein use the 356D/358M allotype, however the other allotype is included herein. That is, any sequence inclusive of an IgG1 Fc domain included herein can have 356E/358L replacing the 356D/358M allotype.

In addition, many of the sequences herein have at least one the cysteines at position 220 replaced by a serine; generally this is the on the “scFv monomer” side for most of the sequences depicted herein, although it can also be on the “Fab monomer” side, or both, to reduce disulfide formation. Specifically included within the sequences herein are one or both of these cysteines replaced (C220S).

Thus, “isotype” as used herein is meant any of the subclasses of immunoglobulins defined by the chemical and antigenic characteristics of their constant regions. It should be understood that therapeutic antibodies can also comprise hybrids of isotypes and/or subclasses. For example, as shown in US Publication 2009/0163699, incorporated by reference, the present invention covers pI engineering of IgG1/G2 hybrids.

The amino-terminal portion of each chain includes a variable region of about 100 to 110 or more amino acids primarily responsible for antigen recognition, generally referred to in the art and herein as the “Fv domain” or “Fv region”. In the variable region, three loops are gathered for each of the V domains of the heavy chain and light chain to form an antigen-binding site. Each of the loops is referred to as a complementarity-determining region (hereinafter referred to as a “CDR”), in which the variation in the amino acid sequence is most significant. “Variable” refers to the fact that certain segments of the variable region differ extensively in sequence among antibodies. Variability within the variable region is not evenly distributed. Instead, the V regions consist of relatively invariant stretches called framework regions (FRs) of 15-30 amino acids separated by shorter regions of extreme variability called “hypervariable regions” that are each 9-15 amino acids long or longer.

Each VH and VL is composed of three hypervariable regions (“complementary determining regions,” “CDRs”) and four FRs, arranged from amino-terminus to carboxy-terminus in the following order: FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4.

The hypervariable region generally encompasses amino acid residues from about amino acid residues 24-34 (LCDR1; “L” denotes light chain), 50-56 (LCDR2) and 89-97 (LCDR3) in the light chain variable region and around about 31-35B (HCDR1; “H” denotes heavy chain), 50-65 (HCDR2), and 95-102 (HCDR3) in the heavy chain variable region; Kabat et al., SEQUENCES OF PROTEINS OF IMMUNOLOGICAL INTEREST, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991) and/or those residues forming a hypervariable loop (e.g. residues 26-32 (LCDR1), 50-52 (LCDR2) and 91-96 (LCDR3) in the light chain variable region and 26-32 (HCDR1), 53-55 (HCDR2) and 96-101 (HCDR3) in the heavy chain variable region; Chothia and Lesk (1987) J. Mol. Biol. 196:901-917. Specific CDRs of the invention are described below.

As will be appreciated by those in the art, the exact numbering and placement of the CDRs can be different among different numbering systems. However, it should be understood that the disclosure of a variable heavy and/or variable light sequence includes the disclosure of the associated CDRs. Accordingly, the disclosure of each variable heavy region is a disclosure of the vhCDRs (e.g. vhCDR1, vhCDR2 and vhCDR3) and the disclosure of each variable light region is a disclosure of the vlCDRs (e.g. vlCDR1, vlCDR2 and vlCDR3).

Throughout the present specification, the Kabat numbering system is generally used when referring to a residue in the variable domain (approximately, residues 1-107 of the light chain variable region and residues 1-113 of the heavy chain variable region) and the EU numbering system for Fc regions (e.g, Kabat et al., supra (1991)).

The present invention provides a large number of different CDR sets. In this case, a “full CDR set” comprises the three variable light and three variable heavy CDRs, e.g. a vlCDR1, vlCDR2, vlCDR3, vhCDR1, vhCDR2 and vhCDR3. These can be part of a larger variable light or variable heavy domain, respectfully. In addition, as more fully outlined herein, the variable heavy and variable light domains can be on separate polypeptide chains, when a heavy and light chain is used (for example when Fabs are used), or on a single polypeptide chain in the case of scFv sequences.

The CDRs contribute to the formation of the antigen-binding, or more specifically, epitope binding site of antibodies. “Epitope” refers to a determinant that interacts with a specific antigen binding site in the variable region of an antibody molecule known as a paratope. Epitopes are groupings of molecules such as amino acids or sugar side chains and usually have specific structural characteristics, as well as specific charge characteristics. A single antigen may have more than one epitope.

The epitope may comprise amino acid residues directly involved in the binding (also called immunodominant component of the epitope) and other amino acid residues, which are not directly involved in the binding, such as amino acid residues which are effectively blocked by the specifically antigen binding peptide; in other words, the amino acid residue is within the footprint of the specifically antigen binding peptide.

Epitopes may be either conformational or linear. A conformational epitope is produced by spatially juxtaposed amino acids from different segments of the linear polypeptide chain. A linear epitope is one produced by adjacent amino acid residues in a polypeptide chain. Conformational and nonconformational epitopes may be distinguished in that the binding to the former but not the latter is lost in the presence of denaturing solvents.

An epitope typically includes at least 3, and more usually, at least 5 or 8-10 amino acids in a unique spatial conformation. Antibodies that recognize the same epitope can be verified in a simple immunoassay showing the ability of one antibody to block the binding of another antibody to a target antigen, for example “binning.”

The carboxy-terminal portion of each chain defines a constant region primarily responsible for effector function. Kabat et al. collected numerous primary sequences of the variable regions of heavy chains and light chains. Based on the degree of conservation of the sequences, they classified individual primary sequences into the CDR and the framework and made a list thereof (see SEQUENCES OF IMMUNOLOGICAL INTEREST, 5th edition, NIH publication, No. 91-3242, E. A. Kabat et al., entirely incorporated by reference).

In the IgG subclass of immunoglobulins, there are several immunoglobulin domains in the heavy chain. By “immunoglobulin (Ig) domain” herein is meant a region of an immunoglobulin having a distinct tertiary structure. Of interest in the present invention are the heavy chain domains, including, the constant heavy (CH) domains and the hinge domains. In the context of IgG antibodies, the IgG isotypes each have three CH regions. Accordingly, “CH” domains in the context of IgG are as follows: “CH1” refers to positions 118-220 according to the EU index as in Kabat. “CH2” refers to positions 237-340 according to the EU index as in Kabat, and “CH3” refers to positions 341-447 according to the EU index as in Kabat. As shown herein and described below, the pI variants can be in one or more of the CH regions, as well as the hinge region, discussed below.

It should be noted that the sequences depicted herein start at the CH1 region, position 118; the variable regions are not included except as noted. For example, the first amino acid of SEQ ID NO: 2, while designated as position“1” in the sequence listing, corresponds to position 118 of the CH1 region, according to EU numbering.

Another type of Ig domain of the heavy chain is the hinge region. By “hinge” or “hinge region” or “antibody hinge region” or “immunoglobulin hinge region” herein is meant the flexible polypeptide comprising the amino acids between the first and second constant domains of an antibody. Structurally, the IgG CH1 domain ends at EU position 220, and the IgG CH2 domain begins at residue EU position 237. Thus for IgG the antibody hinge is herein defined to include positions 221 (D221 in IgG1) to 236 (G236 in IgG1), wherein the numbering is according to the EU index as in Kabat. In some embodiments, for example in the context of an Fc region, the lower hinge is included, with the “lower hinge” generally referring to positions 226 or 230. As noted herein, pI variants can be made in the hinge region as well.

The light chain generally comprises two domains, the variable light domain (containing the light chain CDRs and together with the variable heavy domains forming the Fv region), and a constant light chain region (often referred to as CL or Cx).

Another region of interest for additional substitutions, outlined below, is the Fc region.

Thus, the present invention provides different antibody domains. As described herein and known in the art, the heterodimeric antibodies of the invention comprise different domains within the heavy and light chains, which can be overlapping as well. These domains include, but are not limited to, the Fc domain, the CH1 domain, the CH2 domain, the CH3 domain, the hinge domain, the heavy constant domain (CH1-hinge-Fc domain or CH1-hinge-CH2-CH3), the variable heavy domain, the variable light domain, the light constant domain, FAb domains and scFv domains.

Thus, the “Fc domain” includes the -CH2-CH3 domain, and optionally a hinge domain. In the embodiments herein, when a scFv is attached to an Fc domain, it is the C-terminus of the scFv construct that is attached to the hinge of the Fc domain; for example, it is generally attached to the sequence EPKS which is the beginning of the hinge. The heavy chain comprises a variable heavy domain and a constant domain, which includes a CH1-optional hinge-Fc domain comprising a CH2-CH3. The light chain comprises a variable light chain and the light constant domain. A scFv comprises a variable heavy chain, an scFv linker, and a variable light domain. In most of the constructs and sequences outlined herein, C-terminus of the variable light chain is attached to the N-terminus of the scFv linker, the C-terminus of which is attached to the N-terminus of a variable heavy chain (N-vh-linker-vl-C) although that can be switched (N-vl-linker-vh-C).

Some embodiments of the invention comprise at least one scFv domain, which, while not naturally occurring, generally includes a variable heavy domain and a variable light domain, linked together by a scFv linker. As shown herein, there are a number of suitable scFv linkers that can be used, including traditional peptide bonds, generated by recombinant techniques.

The linker peptide may predominantly include the following amino acid residues: Gly, Ser, Ala, or Thr. The linker peptide should have a length that is adequate to link two molecules in such a way that they assume the correct conformation relative to one another so that they retain the desired activity. In one embodiment, the linker is from about 1 to 50 amino acids in length, preferably about 1 to 30 amino acids in length. In one embodiment, linkers of 1 to 20 amino acids in length may be used, with from about 5 to about 10 amino acids finding use in some embodiments. Useful linkers include glycine-serine polymers, including for example (GS)n, (GSGGS)n, (GGGGS)n, and (GGGS)n, where n is an integer of at least one (and generally from 3 to 4), glycine-alanine polymers, alanine-serine polymers, and other flexible linkers. Alternatively, a variety of nonproteinaceous polymers, including but not limited to polyethylene glycol (PEG), polypropylene glycol, polyoxyalkylenes, or copolymers of polyethylene glycol and polypropylene glycol, may find use as linkers, that is may find use as linkers.

Other linker sequences may include any sequence of any length of CL/CH1 domain but not all residues of CL/CH1 domain; for example the first 5-12 amino acid residues of the CL/CH1 domains. Linkers can be derived from immunoglobulin light chain, for example Cκ or Cλ. Linkers can be derived from immunoglobulin heavy chains of any isotype, including for example Cγ1, Cγ2, Cγ3, Cγ4, Cα1, Cα2, Cδ, Cε, and Cμ. Linker sequences may also be derived from other proteins such as Ig-like proteins (e.g. TCR, FcR, KIR), hinge region-derived sequences, and other natural sequences from other proteins.

In some embodiments, the linker is a “domain linker”, used to link any two domains as outlined herein together. While any suitable linker can be used, many embodiments utilize a glycine-serine polymer, including for example (GS)n, (GSGGS)n, (GGGGS)n, and (GGGS)n, where n is an integer of at least one (and generally from 3 to 4 to 5) as well as any peptide sequence that allows for recombinant attachment of the two domains with sufficient length and flexibility to allow each domain to retain its biological function. In some cases, and with attention being paid to “strandedness”, as outlined below, charged domain linkers, as used in some embodiments of scFv linkers can be used.

In some embodiments, the scFv linker is a charged scFv linker, a number of which are shown in FIG. 14. Accordingly, the present invention further provides charged scFv linkers, to facilitate the separation in pI between a first and a second monomer. That is, by incorporating a charged scFv linker, either positive or negative (or both, in the case of scaffolds that use scFvs on different monomers), this allows the monomer comprising the charged linker to alter the pI without making further changes in the Fc domains. These charged linkers can be substituted into any scFv containing standard linkers. Again, as will be appreciated by those in the art, charged scFv linkers are used on the correct “strand” or monomer, according to the desired changes in pI. For example, as discussed herein, to make triple F format heterodimeric antibody, the original pI of the Fv region for each of the desired antigen binding domains are calculated, and one is chosen to make an scFv, and depending on the pI, either positive or negative linkers are chosen.

Charged domain linkers can also be used to increase the pI separation of the monomers of the invention as well, and thus those included in FIG. 14 can be used in any embodiment herein where a linker is utilized.

In some embodiments, the antibodies are full length. By “full length antibody” herein is meant the structure that constitutes the natural biological form of an antibody, including variable and constant regions, including one or more modifications as outlined herein, particularly in the Fc domains to allow either heterodimerization formation or the purification of heterodimers away from homodimers. Full length antibodies generally include Fab and Fc domains, and can additionally contain extra antigen binding domains such as scFvs, as is generally depicted in the FIG. 1.

In one embodiment, the antibody is an antibody fragment, as long as it contains at least one constant domain which can be engineered to produce heterodimers, such as pI engineering. Other antibody fragments that can be used include fragments that contain one or more of the CH1, CH2, CH3, hinge and CL domains of the invention that have been pI engineered. For example, Fc fusions are fusions of the Fc region (CH2 and CH3, optionally with the hinge region) fused to another protein. A number of Fc fusions are known the art and can be improved by the addition of the heterodimerization variants of the invention. In the present case, antibody fusions can be made comprising CH1; CH1, CH2 and CH3; CH2; CH3; CH2 and CH3; CH1 and CH3, any or all of which can be made optionally with the hinge region, utilizing any combination of heterodimerization variants described herein.

In particular, the formats depicted in FIG. 1 are antibodies, usually referred to as “heterodimeric antibodies”, meaning that the protein has at least two associated Fc sequences self-assembled into a heterodimeric Fc domain.

Chimeric and Humanized Antibodies

In some embodiments, the antibody can be a mixture from different species, e.g. a chimeric antibody and/or a humanized antibody. In general, both “chimeric antibodies” and “humanized antibodies” refer to antibodies that combine regions from more than one species. For example, “chimeric antibodies” traditionally comprise variable region(s) from a mouse (or rat, in some cases) and the constant region(s) from a human. “Humanized antibodies” generally refer to non-human antibodies that have had the variable-domain framework regions swapped for sequences found in human antibodies. Generally, in a humanized antibody, the entire antibody, except the CDRs, is encoded by a polynucleotide of human origin or is identical to such an antibody except within its CDRs. The CDRs, some or all of which are encoded by nucleic acids originating in a non-human organism, are grafted into the beta-sheet framework of a human antibody variable region to create an antibody, the specificity of which is determined by the engrafted CDRs. The creation of such antibodies is described in, e.g., WO 92/11018, Jones, 1986, Nature 321:522-525, Verhoeyen et al., 1988, Science 239:1534-1536, all entirely incorporated by reference. “Backmutation” of selected acceptor framework residues to the corresponding donor residues is often required to regain affinity that is lost in the initial grafted construct (U.S. Pat. No. 5,530,101; U.S. Pat. No. 5,585,089; U.S. Pat. No. 5,693,761; U.S. Pat. No. 5,693,762; U.S. Pat. No. 6,180,370; U.S. Pat. No. 5,859,205; U.S. Pat. No. 5,821,337; U.S. Pat. No. 6,054,297; U.S. Pat. No. 6,407,213, all entirely incorporated by reference). The humanized antibody optimally also will comprise at least a portion of an immunoglobulin constant region, typically that of a human immunoglobulin, and thus will typically comprise a human Fc region. Humanized antibodies can also be generated using mice with a genetically engineered immune system. Roque et al., 2004, Biotechnol. Prog. 20:639-654, entirely incorporated by reference. A variety of techniques and methods for humanizing and reshaping non-human antibodies are well known in the art (See Tsurushita & Vasquez, 2004, Humanization of Monoclonal Antibodies, Molecular Biology of B Cells, 533-545, Elsevier Science (USA), and references cited therein, all entirely incorporated by reference). Humanization methods include but are not limited to methods described in Jones et al., 1986, Nature 321:522-525; Riechmann et al., 1988; Nature 332:323-329; Verhoeyen et al., 1988, Science, 239:1534-1536; Queen et al., 1989, Proc Natl Acad Sci, USA 86:10029-33; He et al., 1998, J. Immunol. 160: 1029-1035; Carter et al., 1992, Proc Natl Acad Sci USA 89:4285-9, Presta et al., 1997, Cancer Res. 57(20):4593-9; Gorman et al., 1991, Proc. Natl. Acad. Sci. USA 88:4181-4185; O'Connor et al., 1998, Protein Eng 11:321-8, all entirely incorporated by reference. Humanization or other methods of reducing the immunogenicity of nonhuman antibody variable regions may include resurfacing methods, as described for example in Roguska et al., 1994, Proc. Natl. Acad. Sci. USA 91:969-973, entirely incorporated by reference.

In certain embodiments, the antibodies of the invention comprise a heavy chain variable region from a particular germline heavy chain immunoglobulin gene and/or a light chain variable region from a particular germline light chain immunoglobulin gene. For example, such antibodies may comprise or consist of a human antibody comprising heavy or light chain variable regions that are “the product of” or “derived from” a particular germline sequence A human antibody that is “the product of” or “derived from” a human germline immunoglobulin sequence can be identified as such by comparing the amino acid sequence of the human antibody to the amino acid sequences of human germline immunoglobulins and selecting the human germline immunoglobulin sequence that is closest in sequence (i.e., greatest % identity) to the sequence of the human antibody. A human antibody that is “the product of” or “derived from” a particular human germline immunoglobulin sequence may contain amino acid differences as compared to the germline sequence, due to, for example, naturally-occurring somatic mutations or intentional introduction of site-directed mutation. However, a humanized antibody typically is at least 90% identical in amino acids sequence to an amino acid sequence encoded by a human germline immunoglobulin gene and contains amino acid residues that identify the antibody as being derived from human sequences when compared to the germline immunoglobulin amino acid sequences of other species (e.g., murine germline sequences). In certain cases, a humanized antibody may be at least 95, 96, 97, 98 or 99%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to the amino acid sequence encoded by the germline immunoglobulin gene. Typically, a humanized antibody derived from a particular human germline sequence will display no more than 10-20 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene (prior to the introduction of any skew, pI and ablation variants herein; that is, the number of variants is generally low, prior to the introduction of the variants of the invention). In certain cases, the humanized antibody may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the amino acid sequence encoded by the germline immunoglobulin gene (again, prior to the introduction of any skew, pI and ablation variants herein; that is, the number of variants is generally low, prior to the introduction of the variants of the invention).

In one embodiment, the parent antibody has been affinity matured, as is known in the art. Structure-based methods may be employed for humanization and affinity maturation, for example as described in U.S. Ser. No. 11/004,590. Selection based methods may be employed to humanize and/or affinity mature antibody variable regions, including but not limited to methods described in Wu et al., 1999, J. Mol. Biol. 294:151-162; Baca et al., 1997, J. Biol. Chem. 272(16):10678-10684; Rosok et al., 1996, J. Biol. Chem. 271(37): 22611-22618; Rader et al., 1998, Proc. Natl. Acad. Sci. USA 95: 8910-8915; Krauss et al., 2003, Protein Engineering 16(10):753-759, all entirely incorporated by reference. Other humanization methods may involve the grafting of only parts of the CDRs, including but not limited to methods described in U.S. Ser. No. 09/810,510; Tan et al., 2002, J. Immunol. 169:1119-1125; De Pascalis et al., 2002, J. Immunol. 169:3076-3084, all entirely incorporated by reference.

IV. Heterodimeric Antibodies

Accordingly, in some embodiments the present invention provides heterodimeric antibodies that rely on the use of two different heavy chain variant Fc domains that will self-assemble to form heterodimeric antibodies.

The present invention is directed to novel constructs to provide heterodimeric antibodies that allow binding to more than one antigen or ligand, e.g. to allow for bispecific binding. The heterodimeric antibody constructs are based on the self-assembling nature of the two Fc domains of the heavy chains of antibodies, e.g. two “monomers” that assemble into a “dimer”. Heterodimeric antibodies are made by altering the amino acid sequence of each monomer as more fully discussed below. Thus, the present invention is generally directed to the creation of heterodimeric antibodies which can co-engage antigens in several ways, relying on amino acid variants in the constant regions that are different on each chain to promote heterodimeric formation and/or allow for ease of purification of heterodimers over the homodimers.

Thus, the present invention provides bispecific antibodies. An ongoing problem in antibody technologies is the desire for “bispecific” antibodies that bind to two different antigens simultaneously, in general thus allowing the different antigens to be brought into proximity and resulting in new functionalities and new therapies. In general, these antibodies are made by including genes for each heavy and light chain into the host cells. This generally results in the formation of the desired heterodimer (A-B), as well as the two homodimers (A-A and B-B (not including the light chain heterodimeric issues)). However, a major obstacle in the formation of bispecific antibodies is the difficulty in purifying the heterodimeric antibodies away from the homodimeric antibodies and/or biasing the formation of the heterodimer over the formation of the homodimers.

There are a number of mechanisms that can be used to generate the heterodimers of the present invention. In addition, as will be appreciated by those in the art, these mechanisms can be combined to ensure high heterodimerization. Thus, amino acid variants that lead to the production of heterodimers are referred to as “heterodimerization variants”. As discussed below, heterodimerization variants can include steric variants (e.g. the “knobs and holes” or “skew” variants described below and the “charge pairs” variants described below) as well as “pI variants”, which allows purification of homodimers away from heterodimers. As is generally described in WO2014/145806, hereby incorporated by reference in its entirety and specifically as below for the discussion of “heterodimerization variants”, useful mechanisms for heterodimerization include “knobs and holes” (“KIH”; sometimes herein as “skew” variants (see discussion in WO2014/145806), “electrostatic steering” or “charge pairs” as described in WO2014/145806, pI variants as described in WO2014/145806, and general additional Fc variants as outlined in WO2014/145806 and below.

In the present invention, there are several basic mechanisms that can lead to ease of purifying heterodimeric antibodies; one relies on the use of pI variants, such that each monomer has a different pI, thus allowing the isoelectric purification of A-A, A-B and B-B dimeric proteins. Alternatively, some scaffold formats, such as the “triple F” format, also allows separation on the basis of size. As is further outlined below, it is also possible to “skew” the formation of heterodimers over homodimers. Thus, a combination of steric heterodimerization variants and pI or charge pair variants find particular use in the invention.

In general, embodiments of particular use in the present invention rely on sets of variants that include skew variants, that encourage heterodimerization formation over homodimerization formation, coupled with pI variants, which increase the pI difference between the two monomers.

Additionally, as more fully outlined below, depending on the format of the heterodimer antibody, pI variants can be either contained within the constant and/or Fc domains of a monomer, or charged linkers, either domain linkers or scFv linkers, can be used. That is, scaffolds that utilize scFv(s) such as the Triple F format can include charged scFv linkers (either positive or negative), that give a further pI boost for purification purposes. As will be appreciated by those in the art, some Triple F formats are useful with just charged scFv linkers and no additional pI adjustments, although the invention does provide pI variants that are on one or both of the monomers, and/or charged domain linkers as well. In addition, additional amino acid engineering for alternative functionalities may also confer pI changes, such as Fc, FcRn and KO variants.

In the present invention that utilizes pI as a separation mechanism to allow the purification of heterodimeric proteins, amino acid variants can be introduced into one or both of the monomer polypeptides; that is, the pI of one of the monomers (referred to herein for simplicity as “monomer A”) can be engineered away from monomer B, or both monomer A and B change be changed, with the pI of monomer A increasing and the pI of monomer B decreasing. As is outlined more fully below, the pI changes of either or both monomers can be done by removing or adding a charged residue (e.g. a neutral amino acid is replaced by a positively or negatively charged amino acid residue, e.g. glycine to glutamic acid), changing a charged residue from positive or negative to the opposite charge (aspartic acid to lysine) or changing a charged residue to a neutral residue (e.g. loss of a charge; lysine to serine.). A number of these variants are shown in the Figures.

Accordingly, this embodiment of the present invention provides for creating a sufficient change in pI in at least one of the monomers such that heterodimers can be separated from homodimers. As will be appreciated by those in the art, and as discussed further below, this can be done by using a “wild type” heavy chain constant region and a variant region that has been engineered to either increase or decrease it's pI (wt A−+B or wt A−−B), or by increasing one region and decreasing the other region (A+−B− or A−B+).

Thus, in general, a component of some embodiments of the present invention are amino acid variants in the constant regions of antibodies that are directed to altering the isoelectric point (pI) of at least one, if not both, of the monomers of a dimeric protein to form “pI antibodies”) by incorporating amino acid substitutions (“pI variants” or “pI substitutions”) into one or both of the monomers. As shown herein, the separation of the heterodimers from the two homodimers can be accomplished if the pIs of the two monomers differ by as little as 0.1 pH unit, with 0.2, 0.3, 0.4 and 0.5 or greater all finding use in the present invention.

As will be appreciated by those in the art, the number of pI variants to be included on each or both monomer(s) to get good separation will depend in part on the starting pI of the components, for example in the triple F format, the starting pI of the scFv and Fab of interest. That is, to determine which monomer to engineer or in which “direction” (e.g. more positive or more negative), the Fv sequences of the two target antigens are calculated and a decision is made from there. As is known in the art, different Fvs will have different starting pIs which are exploited in the present invention. In general, as outlined herein, the pIs are engineered to result in a total pI difference of each monomer of at least about 0.1 logs, with 0.2 to 0.5 being preferred as outlined herein.

Furthermore, as will be appreciated by those in the art and outlined herein, in some embodiments, heterodimers can be separated from homodimers on the basis of size. As shown in FIG. 1 for example, several of the formats allow separation of heterodimers and homodimers on the basis of size.

In the case where pI variants are used to achieve heterodimerization, by using the constant region(s) of the heavy chain(s), a more modular approach to designing and purifying bispecific proteins, including antibodies, is provided. Thus, in some embodiments, heterodimerization variants (including skew and purification heterodimerization variants) are not included in the variable regions, such that each individual antibody must be engineered. In addition, in some embodiments, the possibility of immunogenicity resulting from the pI variants is significantly reduced by importing pI variants from different IgG isotypes such that pI is changed without introducing significant immunogenicity. Thus, an additional problem to be solved is the elucidation of low pI constant domains with high human sequence content, e.g. the minimization or avoidance of non-human residues at any particular position.

A side benefit that can occur with this pI engineering is also the extension of serum half-life and increased FcRn binding. That is, as described in U.S. Ser. No. 13/194,904 (incorporated by reference in its entirety), lowering the pI of antibody constant domains (including those found in antibodies and Fc fusions) can lead to longer serum retention in vivo. These pI variants for increased serum half life also facilitate pI changes for purification.

In addition, it should be noted that the pI variants of the heterodimerization variants give an additional benefit for the analytics and quality control process of bispecific antibodies, as the ability to either eliminate, minimize and distinguish when homodimers are present is significant. Similarly, the ability to reliably test the reproducibility of the heterodimeric antibody production is important.

Heterodimerization Variants

The present invention provides heterodimeric proteins, including heterodimeric antibodies in a variety of formats, which utilize heterodimeric variants to allow for heterodimeric formation and/or purification away from homodimers.

There are a number of suitable pairs of sets of heterodimerization skew variants. These variants come in “pairs” of “sets”. That is, one set of the pair is incorporated into the first monomer and the other set of the pair is incorporated into the second monomer. It should be noted that these sets do not necessarily behave as “knobs in holes” variants, with a one-to-one correspondence between a residue on one monomer and a residue on the other; that is, these pairs of sets form an interface between the two monomers that encourages heterodimer formation and discourages homodimer formation, allowing the percentage of heterodimers that spontaneously form under biological conditions to be over 90%, rather than the expected 50% (25% homodimer A/A:50% heterodimer A/B:25% homodimer B/B).

Steric Variants

In some embodiments, the formation of heterodimers can be facilitated by the addition of steric variants. That is, by changing amino acids in each heavy chain, different heavy chains are more likely to associate to form the heterodimeric structure than to form homodimers with the same Fc amino acid sequences. Suitable steric variants are included in FIG. 10.

One mechanism is generally referred to in the art as “knobs and holes”, referring to amino acid engineering that creates steric influences to favor heterodimeric formation and disfavor homodimeric formation can also optionally be used; this is sometimes referred to as “knobs and holes”, as described in U.S. Ser. No. 61/596,846, Ridgway et al., Protein Engineering 9(7):617 (1996); Atwell et al., J. Mol. Biol. 1997 270:26; U.S. Pat. No. 8,216,805, all of which are hereby incorporated by reference in their entirety. The Figures identify a number of “monomer A-monomer B” pairs that rely on “knobs and holes”. In addition, as described in Merchant et al., Nature Biotech. 16:677 (1998), these “knobs and hole” mutations can be combined with disulfide bonds to skew formation to heterodimerization.

An additional mechanism that finds use in the generation of heterodimers is sometimes referred to as “electrostatic steering” as described in Gunasekaran et al., J. Biol. Chem. 285(25):19637 (2010), hereby incorporated by reference in its entirety. This is sometimes referred to herein as “charge pairs”. In this embodiment, electrostatics are used to skew the formation towards heterodimerization. As those in the art will appreciate, these may also have have an effect on pI, and thus on purification, and thus could in some cases also be considered pI variants. However, as these were generated to force heterodimerization and were not used as purification tools, they are classified as “steric variants”. These include, but are not limited to, D221E/P228E/L368E paired with D221R/P228R/K409R (e.g. these are “monomer corresponding sets) and C220E/P228E/368E paired with C220R/E224R/P228R/K409R.

Additional monomer A and monomer B variants that can be combined with other variants, optionally and independently in any amount, such as pI variants outlined herein or other steric variants that are shown in FIG. 37 of US 2012/0149876, the figure and legend and SEQ ID NOs of which are incorporated expressly by reference herein.

In some embodiments, the steric variants outlined herein can be optionally and independently incorporated with any pI variant (or other variants such as Fc variants, FcRn variants, etc.) into one or both monomers, and can be independently and optionally included or excluded from the proteins of the invention.

A list of suitable skew variants is found in FIG. 10, with FIG. 15 showing some pairs of particular utility in many embodiments. Of particular use in many embodiments are the pairs of sets including, but not limited to, Particularly preferred steric variant pairs include, but are not limited to, L368D/K370S: S364K, L368E/K370S: S364K, T411E/K360E/Q362E: D401K, L368D/K370S: S364K/E357L, K370S: S364K/E357Q and S364K/E357Q: L368D/K370S. In terms of nomenclature, the pair “S364K/E357Q: L368D/K370S” means that one of the monomers has the double variant set S364K/E357Q and the other has the double variant set L368D/K370S.

pI (Isoelectric Point) Variants for Heterodimers

In general, as will be appreciated by those in the art, there are two general categories of pI variants: those that increase the pI of the protein (basic changes) and those that decrease the pI of the protein (acidic changes). As described herein, all combinations of these variants can be done: one monomer may be wild type, or a variant that does not display a significantly different pI from wild-type, and the other can be either more basic or more acidic. Alternatively, each monomer is changed, one to more basic and one to more acidic.

Preferred combinations of pI variants are shown in FIG. 11. As outlined herein and shown in the figures, these changes are shown relative to IgG1, but all isotypes can be altered this way, as well as isotype hybrids. In the case where the heavy chain constant domain is from IgG2-4, R133E and R133Q can also be used.

In one embodiment, for example in the bottle opener format, a preferred combination of pI variants has one monomer (the negative Fab side) comprising 208D/295E/384D/418E/421D variants (N208D/Q295E/N384D/Q418E/N421D when relative to human IgG1) and a second monomer (the positive scFv side) comprising a positively charged scFv linker, including (GKPGS)4. However, as will be appreciated by those in the art, the first monomer includes a CH1 domain, including position 208. Accordingly, in constructs that do not include a CH1 domain (for example for heterodimeric Fc fusion proteins that do not utilize a CH1 domain on one of the domains, for example in a dual scFv format), a preferred negative pI variant Fc set includes 295E/384D/418E/421D variants (Q295E/N384D/Q418E/N421D when relative to human IgG1).

Antibody Heterodimers Light Chain Variants

In the case of antibody based heterodimers, e.g. where at least one of the monomers comprises a light chain in addition to the heavy chain domain, pI variants can also be made in the light chain. Amino acid substitutions for lowering the pI of the light chain include, but are not limited to, K126E, K126Q, K145E, K145Q, N152D, S156E, K169E, S202E, K207E and adding peptide DEDE at the c-terminus of the light chain. Changes in this category based on the constant lambda light chain include one or more substitutions at R108Q Q124E, K126Q, N138D, K145T and Q199E. In addition, increasing the pI of the light chains can also be done.

Isotypic Variants

In addition, many embodiments of the invention rely on the “importation” of pI amino acids at particular positions from one IgG isotype into another, thus reducing or eliminating the possibility of unwanted immunogenicity being introduced into the variants. A number of these are shown in FIG. 21 of US Publ. 2014/0370013, hereby incorporated by reference. That is, IgG1 is a common isotype for therapeutic antibodies for a variety of reasons, including high effector function. However, the heavy constant region of IgG1 has a higher pI than that of IgG2 (8.10 versus 7.31). By introducing IgG2 residues at particular positions into the IgG1 backbone, the pI of the resulting monomer is lowered (or increased) and additionally exhibits longer serum half-life. For example, IgG1 has a glycine (pI 5.97) at position 137, and IgG2 has a glutamic acid (pI 3.22); importing the glutamic acid will affect the pI of the resulting protein. As is described below, a number of amino acid substitutions are generally required to significant affect the pI of the variant antibody. However, it should be noted as discussed below that even changes in IgG2 molecules allow for increased serum half-life.

In other embodiments, non-isotypic amino acid changes are made, either to reduce the overall charge state of the resulting protein (e.g. by changing a higher pI amino acid to a lower pI amino acid), or to allow accommodations in structure for stability, etc. as is more further described below.

In addition, by pI engineering both the heavy and light constant domains, significant changes in each monomer of the heterodimer can be seen. As discussed herein, having the pIs of the two monomers differ by at least 0.5 can allow separation by ion exchange chromatography or isoelectric focusing, or other methods sensitive to isoelectric point.

Calculating pI

The pI of each monomer can depend on the pI of the variant heavy chain constant domain and the pI of the total monomer, including the variant heavy chain constant domain and the fusion partner. Thus, in some embodiments, the change in pI is calculated on the basis of the variant heavy chain constant domain, using the chart in the FIG. 19 of US Pub. 2014/0370013. As discussed herein, which monomer to engineer is generally decided by the inherent pI of the Fv and scaffold regions. Alternatively, the pI of each monomer can be compared.

pI Variants That Also Confer Better FcRn In Vivo Binding

In the case where the pI variant decreases the pI of the monomer, they can have the added benefit of improving serum retention in vivo.

Although still under examination, Fc regions are believed to have longer half-lives in vivo, because binding to FcRn at pH 6 in an endosome sequesters the Fc (Ghetie and Ward, 1997 Immunol Today. 18(12): 592-598, entirely incorporated by reference). The endosomal compartment then recycles the Fc to the cell surface. Once the compartment opens to the extracellular space, the higher pH, -7.4, induces the release of Fc back into the blood. In mice, Dall' Acqua et al. showed that Fc mutants with increased FcRn binding at pH 6 and pH 7.4 actually had reduced serum concentrations and the same half life as wild-type Fc (Dall' Acqua et al. 2002, J. Immunol. 169:5171-5180, entirely incorporated by reference). The increased affinity of Fc for FcRn at pH 7.4 is thought to forbid the release of the Fc back into the blood. Therefore, the Fc mutations that will increase Fc's half-life in vivo will ideally increase FcRn binding at the lower pH while still allowing release of Fc at higher pH. The amino acid histidine changes its charge state in the pH range of 6.0 to 7.4. Therefore, it is not surprising to find His residues at important positions in the Fc/FcRn complex.

Recently it has been suggested that antibodies with variable regions that have lower isoelectric points may also have longer serum half-lives (Igawa et al., 2010 PEDS. 23(5): 385-392, entirely incorporated by reference). However, the mechanism of this is still poorly understood. Moreover, variable regions differ from antibody to antibody. Constant region variants with reduced pI and extended half-life would provide a more modular approach to improving the pharmacokinetic properties of antibodies, as described herein.

Additional Fc Variants for Additional Functionality

In addition to pI amino acid variants, there are a number of useful Fc amino acid modification that can be made for a variety of reasons, including, but not limited to, altering binding to one or more FcγR receptors, altered binding to FcRn receptors, etc.

Accordingly, the proteins of the invention can include amino acid modifications, including the heterodimerization variants outlined herein, which includes the pI variants and steric variants. Each set of variants can be independently and optionally included or excluded from any particular heterodimeric protein.

FcγR Variants

Accordingly, there are a number of useful Fc substitutions that can be made to alter binding to one or more of the FcγR receptors. Substitutions that result in increased binding as well as decreased binding can be useful. For example, it is known that increased binding to Fc

RIIIa generally results in increased ADCC (antibody dependent cell-mediated cytotoxicity; the cell-mediated reaction wherein nonspecific cytotoxic cells that express FcγRs recognize bound antibody on a target cell and subsequently cause lysis of the target cell). Similarly, decreased binding to FcγRIIb (an inhibitory receptor) can be beneficial as well in some circumstances. Amino acid substitutions that find use in the present invention include those listed in U.S. Ser. No. 11/124,620 (particularly FIG. 41), Ser. Nos. 11/174,287, 11/396,495, 11/538,406, all of which are expressly incorporated herein by reference in their entirety and specifically for the variants disclosed therein. Particular variants that find use include, but are not limited to, 236A, 239D, 239E, 332E, 332D, 239D/332E, 267D, 267E, 328F, 267E/328F, 236A/332E, 239D/332E/330Y, 239D, 332E/330L, 243A, 243L, 264A, 264V and 299T.

In addition, there are additional Fc substitutions that find use in increased binding to the FcRn receptor and increased serum half life, as specifically disclosed in U.S. Ser. No. 12/341,769, hereby incorporated by reference in its entirety, including, but not limited to, 434S, 434A, 428L, 308F, 2591, 428L/4345, 2591/308F, 4361/428L, 4361 or V/4345, 436V/428L and 2591/308F/428L.

Ablation Variants

Similarly, another category of functional variants are “FcγR ablation variants” or “Fc knock out (FcKO or KO)” variants. In these embodiments, for some therapeutic applications, it is desirable to reduce or remove the normal binding of the Fc domain to one or more or all of the Fcγ receptors (e.g. FcγR1, FcγRIIa, FcγRIIb, FcγRIIIa, etc.) to avoid additional mechanisms of action. That is, for example, in many embodiments, particularly in the use of bispecific antibodies that bind CD3 monovalently it is generally desirable to ablate FcγRIIIa binding to eliminate or significantly reduce ADCC activity. wherein one of the Fc domains comprises one or more Fcγ receptor ablation variants. These ablation variants are depicted in FIG. 12, and each can be independently and optionally included or excluded, with preferred aspects utilizing ablation variants selected from the group consisting of G236R/L328R, E233P/L234V/L235A/G236del/5239K, E233P/L234V/L235A/G236del/5267K, E233P/L234V/L235A/G236del/5239K/A327G, E233P/L234V/L235A/G236del/5267K/A327G and E233P/L234V/L235A/G236del. It should be noted that the ablation variants referenced herein ablate FcγR binding but generally not FcRn binding.

Combination of Heterodimeric and Fc Variants

As will be appreciated by those in the art, all of the recited heterodimerization variants (including skew and/or pI variants) can be optionally and independently combined in any way, as long as they retain their “strandedness” or “monomer partition”. In addition, all of these variants can be combined into any of the heterodimerization formats.

In the case of pI variants, while embodiments finding particular use are shown in the Figures, other combinations can be generated, following the basic rule of altering the pI difference between two monomers to facilitate purification.

In addition, any of the heterodimerization variants, skew and pI, are also independently and optionally combined with Fc ablation variants, Fc variants, FcRn variants, as generally outlined herein.

Useful Formats of the Invention

As will be appreciated by those in the art and discussed more fully below, the heterodimeric fusion proteins of the present invention can take on a wide variety of configurations, as are generally depicted in FIG. 1. Some figures depict “single ended” configurations, where there is one type of specificity on one “arm” of the molecule and a different specificity on the other “arm”. Other figures depict “dual ended” configurations, where there is at least one type of specificity at the “top” of the molecule and one or more different specificities at the “bottom” of the molecule. Thus, the present invention is directed to novel immunoglobulin compositions that co-engage a different first and a second antigen.

As will be appreciated by those in the art, the heterodimeric formats of the invention can have different valencies as well as be bispecific. That is, heterodimeric antibodies of the invention can be bivalent and bispecific, wherein CD3 is bound by one binding domain and PSMA is bound by a second binding domain. The heterodimeric antibodies can also be trivalent and bispecific, wherein the PSMA is bound by two binding domains and the CD3 by a second binding domain. As is outlined herein, it is preferable that the CD3 is bound only monovalently, to reduce potential side effects.

The present invention utilizes anti-CD3 antigen binding domains and anti-PSMA antigen binding domains. As will be appreciated by those in the art, any collection of anti-CD3 CDRs, anti-CD3 variable light and variable heavy domains, Fabs and scFvs as depicted in any of the Figures (see particularly FIGS. 2 through 7 and 21) can be used.

In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the variable light and variable heavy domains of anti-CD3 H1.30_L1.47 (FIG. 2). In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the vhCDR1-3 and vlCDR1-3 of anti-CD3 H1.30_L1.47 (FIG. 2). In some embodiments, the subject antibody includes an anti-CD3 _H1.30_L1.47 scFv (FIG. 2).

In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the variable light and variable heavy domains of anti-CD3 H1.32_L1.47 (FIG. 3). In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the vhCDR1-3 and vlCDR1-3 of anti-CD3 H1.32_L1.47 (FIG. 3). In some embodiments, the subject antibody includes an anti-CD3 H1.32_L1.47 scFv (FIG. 3).

In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the variable light and variable heavy domains of anti-CD3 H1.89_L1.47 (FIG. 4). In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the vhCDR1-3 and vlCDR1-3 of anti-CD3 H1.89_L1.47 (FIG. 4). In some embodiments, the subject antibody includes an anti-CD3 H1.89_L1.47 scFv (FIG. 4).

In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the variable light and variable heavy domains of anti-CD3 H1.90_L1.47 (FIG. 5). In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the vhCDR1-3 and vlCDR1-3 of anti-CD3 H1.90_L1.47 (FIG. 5). In some embodiments, the subject antibody includes an anti-CD3 H1.90_L1.47 scFv (FIG. 5).

In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the variable light and variable heavy domain of anti-CD3 H1.33_L1.47 (FIG. 6). In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the vhCDR1-3 and vlCDR1-3 of anti-CD3 H1.33_L1.47 (FIG. 6). In some embodiments, the subject antibody includes an anti-CD3 H1.33_L1.47 scFv (FIG. 6).

In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the variable light and variable heavy domain of anti-CD3 H1.31_L1.47 (FIG. 7). In some embodiments, the subject antibody includes an anti-CD3 antigen binding domain that includes the vhCDR1-3 and vlCDR1-3 of anti-CD3 H1.31_L1.47 (FIG. 7). In some embodiments, the subject antibody includes an anti-CD3 H1.31_L1.47 scFv (FIG. 7).

Similarly, any of the anti-PSMA antigen binding domains, whether anti-PSMA CDRs, anti-PSMA variable light and variable heavy domains, Fabs and scFvs as depicted in FIGS. 8 and 20 can be used, optionally and independently combined in any combination. In some embodiments, the subject antibody includes an anti-PSMA antigen binding domain that includes the variable light and variable heavy domains of anti-PSMA J591 H1_L1 (FIG. 20). In some embodiments, the subject antibody includes an anti-PSMA antigen binding domain that includes the vhCDR1-3 and vlCDR1-3 of anti-PSMA J591 H1_L1 (FIG. 20). In some embodiments, the subject antibody includes an anti-PSMA J591 H1_L1 scFv having the variable light and variable heavy domains or vhCDR1-3 and vlCDR1-3 of anti-PSMA J591 H1_11 (FIG. 20).

Bottle Opener Format

One heterodimeric scaffold that finds particular use in the present invention is the “triple F” or “bottle opener” scaffold format as shown in FIG. 1A. In this embodiment, one heavy chain of the antibody contains an single chain Fv (“scFv”, as defined below) and the other heavy chain is a “regular” FAb format, comprising a variable heavy chain and a light chain. This structure is sometimes referred to herein as “triple F” format (scFv-FAb-Fc) or the “bottle-opener” format, due to a rough visual similarity to a bottle-opener (see FIG. 1). The two chains are brought together by the use of amino acid variants in the constant regions (e.g. the Fc domain, the CH1 domain and/or the hinge region) that promote the formation of heterodimeric antibodies as is described more fully below.

There are several distinct advantages to the present “triple F” format. As is known in the art, antibody analogs relying on two scFv constructs often have stability and aggregation problems, which can be alleviated in the present invention by the addition of a “regular” heavy and light chain pairing. In addition, as opposed to formats that rely on two heavy chains and two light chains, there is no issue with the incorrect pairing of heavy and light chains (e.g. heavy 1 pairing with light 2, etc.).

Many of the embodiments outlined herein rely in general on the bottle opener format that comprises a first monomer comprising an scFv, comprising a variable heavy and a variable light domain, covalently attached using an scFv linker (charged, in many instances), where the scFv is covalently attached to the N-terminus of a first Fc domain usually through a domain linker (which, as outlined herein can either be un-charged or charged). The second monomer of the bottle opener format is a heavy chain, and the composition further comprises a light chain.

In general, in many preferred embodiments, the scFv is the domain that binds to the CD3, with the Fab of the heavy and light chains binding to PSMA. In addition, the Fc domains of the invention generally comprise skew variants (e.g. a set of amino acid substitutions as shown in FIGS. 10 and 15, with particularly useful skew variants being selected from the group consisting of S364K/E357Q: L368D/K370S; L368D/K370S: S364K; L368E/K370S: S364K; T411T/E360E/Q362E: D401K; L368D/K370S: S364K/E357L and K370S: S364K/E357Q), optionally ablation variants, and the heavy chain comprises pI variants.

In some embodiments, any of the vh and vl sequences depicted herein (including all anti-PSMA and anti-CD3 vh and vl sequences and related sCDR equences depicted in the Figure) can be added to the bottle opener backbone formats of FIG. 25 as the “Fab side”, using any of the anti-CD3 scFv sequences shown in the Figures. Anti-CD3 sequences finding particular use in these embodiments are anti-CD3 H1.30_11.47, anti-CD3 H1.32_L1.47, anti-CD3 H1.89_L1.47, anti-CD3 H1.90_L1.47, anti-CD3 H1.33_L1.47 and anti-CD3 H1.31_L1.47 (vh, vl and CDR sequences depicted in FIGS. 2,-7, respectively), attached as the scFv side of the backbones shown in FIG. 25.

The present invention provides bottle opener formats where the anti-CD3 scFv sequences are as shown in FIGS. 2 to 7 and 21, including any combination with the backbone formats of FIG. 25. In addition, any of the anti-CD3 vh, vl and CDR sequences as shown in FIGS. 2 to 7 and 21 can be used as the Fab side.

The present invention provides bottle opener formats wherein the anti-PSMA sequences are as shown in FIGS. 8 and 20. Similar to the above, each anti-PSMA vh, vl, and related CDRs sequences can be either the Fab side or the scFv side, and can be linked as one of the antigen binding domains of a bottle opener format, including those of FIG. 25. When the anti-PSMA sequences are the Fab side, any anti-CD3 scFv sequences and related vh, vl and CDR sequences set forth in the Figures can be used, particularly including anti-CD3 H1.30_L1.47, anti-CD3 H1.32_L1.47, anti-CD3 H1.89_L1.47, anti-CD3 H1.90_L1.47, anti-CD3 H1.33_L1.47 and anti-CD3 H1.31_L1.47, attached as the scFv side of the backbones shown in FIG. 25.

In some embodiments, the subject antibody is a bottle opener format antibody having a Fab-Fc heavy chain scaffold, a scFv-Fc heavy chain scaffold and light chain scaffold as shown in FIG. 21E. Any variable heavy domain, including, but not limited to, any of the variable heavy domains described herein can be operably linked to the Fab-Fc heavy chain scaffold described in FIG. 21E. Any variable light domain, including, but not limited to, any of the variable light domains described herein can be operably linked to the light chain scaffold described in FIG. 21E.

In some embodiments, the variable heavy domain and the variable light domain operably linked to the Fab-Fc heavy chain scaffold and light chain scaffold (FIG. 21E), respectively, are variable heavy and light domains of an anti-PSMA binding domain. In some embodiments, the variable heavy domain and the variable light domain includes the variable light and variable heavy domains of anti-PSMA J591 H1_L1 (FIG. 20). In some embodiments, the variable heavy domain includes the vhCDR1-3 of J591 H1_L1 (FIG. 20) and the variable light domain includes the vlCDR1-3 of J591 H1_L1 (FIG. 20).

In some embodiments, the variable heavy domain and the variable light domain operably linked to the Fab-Fc heavy chain scaffold and light chain scaffold (FIG. 21E), respectively, are variable heavy and light domains of an anti-CD3 binding domain. In some embodiments, the variable heavy domain and the variable light domain includes the variable light and variable heavy domains of anti-CD3 H1.30_L1.47 (FIG. 2), anti-CD3 H1.32_L1.47 (FIG. 3), anti-CD3 H1.89_L1.47 (FIG. 4), H1.90_L1.47 (FIG. 5), H1.33_L1.47 (FIG. 6), or H1.31_L1.47 (FIG. 7). In some embodiments, the variable heavy domain includes the vhCDR1-3 of anti-CD3 H1.30_L1.47 (FIG. 2), anti-CD3 H1.32_L1.47 (FIG. 3), anti-CD3 H1.89_L1.47 (FIG. 4), H1.90_L1.47 (FIG. 5), H1.33_L1.47 (FIG. 6), or H1.31_L1.47 (FIG. 7) and the variable light domain includes the vlCDR1-3 of anti-CD3 H1.30_L1.47 (FIG. 2), anti-CD3 H1.32_L1.47 (FIG. 3), anti-CD3 H1.89_L1.47 (FIG. 4), H1.90_L1.47 (FIG. 5), H1.33_L1.47 (FIG. 6), or H1.31_L1.47 (FIG. 7), respectively (e.g., the variable heavy domain includes the vhCDR1-3 of anti-CD3 H1.30_L1.47 and the variable light domain includes the vlCDR1-3 of anti-CD3 H1.30_L1.47).

In some embodiments, the scFv-Fc heavy chain scaffold (FIG. 21E) is operably linked to an anti-CD3 scFv. In some embodiments, the anti-CD3 scFv includes the variable heavy and variable light domains of H1.30_L1.47 (FIG. 2), H1.32_L1.47 (FIG. 3), H1.89_L1.47 (FIG. 4), H1.90_L1.47 (FIG. 5), H1.33_L1.47 (FIG. 6) or H1.31_L1.47 (FIG. 7). In some embodiments, the anti-CD3 scFv includes the vhCDR1-3 and vlCDR1-3 of H1.30_L1.47 (FIG. 2), H1.32_L1.47 (FIG. 3), H1.89_L1.47 (FIG. 4), H1.90_L1.47 (FIG. 5), H1.33_L1.47 (FIG. 6) or H1.31_L1.47 (FIG. 7). In some embodiments, the anti-CD3 scFv is an H1.30_L1.47 scFv (FIG. 2), H1.32_L1.47 scFv (FIG. 3), H1.89_L1.47 scFv (FIG. 4), H1.90_L1.47 scFv (FIG. 5), H1.33_L1.47 scFv (FIG. 6) or H1.31_L1.47 scFv (FIG. 7)

In some embodiments, the scFv-Fc heavy chain scaffold (FIG. 21E) is operably linked to an anti-PSMA scFv. In some embodiments, the anti-PSMA scFv includes the variable heavy domain and variable light domain of anti-PSMA J591 H1_L1 (FIG. 20). In some embodiments, the scFv includes the vhCDR1-3 of J591 H1_L1 (FIG. 20) and the vlCDR1-3 of J591 H1_L1 (FIG. 20). In some embodiments the anti-PSMA scFv is an J591 H1_L1 scFv (FIG. 20).

In certain embodiments, the subject anti-PSMA x anti x CD3 antibody has the sequence of any one of the bottle opener formats depicted in FIGS. 9, 21A-D and 25.

mAb-Fv Format

One heterodimeric scaffold that finds particular use in the present invention is the mAb-Fv format shown in FIG. 1. In this embodiment, the format relies on the use of a C-terminal attachment of an “extra” variable heavy domain to one monomer and the C-terminal attachment of an “extra” variable light domain to the other monomer, thus forming a third antigen binding domain, wherein the Fab portions of the two monomers bind PSMA and the “extra” Fv domain binds CD3.

In this embodiment, the first monomer comprises a first heavy chain, comprising a first variable heavy domain and a first constant heavy domain comprising a first Fc domain, with a first variable light domain covalently attached to the C-terminus of the first Fc domain using a domain linker. The second monomer comprises a second variable heavy domain, a second constant heavy domain comprising a second Fc domain, and a third variable heavy domain covalently attached to the C-terminus of the second Fc domain using a domain linker. In some embodiments, the two C-terminally attached variable domains make up a Fv that binds CD3 (as it is less preferred to have bivalent CD3 binding). This embodiment further utilizes a common light chain comprising a variable light domain and a constant light domain that associates with the heavy chains to form two identical Fabs that bind PSMA.

In some embodiments, the first heavy chain includes vh1-CH1-hinge-CH2-CH3-[optional linker]-v12 and the second monomer includes vh1-CH1-hinge-CH2-CH3-[optional linker]-vh2 and the common light chain includes v11. In this embodiment, vh1 and v11 form PSMA binding domains and vh2 and v12 form a CD3 binding domain.

As for many of the embodiments herein, these constructs include skew variants, pI variants, ablation variants, additional Fc variants, etc. as desired and described herein.

The present invention provides mAb-Fv formats where the anti-CD3 scFv sequences are shown in FIG. 2 to FIG. 7.

The present invention provides bottle opener formats wherein the anti-PSMA sequences are as shown in FIG. 8 and FIG. 20.

The present invention provides mAb-Fv formats comprising ablation variants as shown in FIG. 12.

The present invention provides mAb-Fv formats comprising skew variants as shown in FIG. 10 and FIG. 15.

mAb-scFv

One heterodimeric scaffold that finds particular use in the present invention is the mAb-scFv format shown in FIG. 1. In this embodiment, the format relies on the use of a C-terminal attachment of a scFv to one of the monomers, thus forming a third antigen binding domain, wherein the Fab portions of the two monomers bind PSMA and the “extra” scFv domain binds CD3. Thus, the first monomer comprises a first heavy chain (comprising a variable heavy domain and a constant domain), with a C-terminally covalently attached scFv comprising a scFv variable light domain, an scFv linker and a scFv variable heavy domain in either orientation (vh1-CH1-hinge-CH2-CH3-[optional linker]-vh2-scFv linker-vl2 or vh1-CH1-hinge-CH2-CH3-[optional linker]-vl2-scFv linker-vh2). This embodiment further utilizes a common light chain comprising a variable light domain and a constant light domain that associates with the heavy chains to form two identical Fabs that bind PSMA. As for many of the embodiments herein, these constructs include skew variants, pI variants, ablation variants, additional Fc variants, etc. as desired and described herein.

The present invention provides mAb-scFv formats where the anti-CD3 scFv sequences are as shown in FIG. 2 to FIG. 7.

The present invention provides mAb-scFv formats wherein the anti-PSMA sequences are as shown in FIG. 8 and FIG. 20.

The present invention provides mAb-scFv formats comprising ablation variants as shown in FIG. 12.

The present invention provides mAb-scFv formats comprising skew variants as shown in FIG. 10 and FIG. 15.

Central scFv Format

One heterodimeric scaffold that finds particular use in the present invention is the Central-scFv format shown in FIG. 1. In this embodiment, the format relies on the use of an inserted scFv domain thus forming a third antigen binding domain, wherein the Fab portions of the two monomers bind PSMA and the “extra” scFv domain binds CD3. The scFv domain is inserted between the Fc domain and the CH1-Fv region of one of the monomers, thus providing a third antigen binding domain.

In this embodiment, one monomer comprises a first heavy chain comprising a first variable heavy domain, a CH1 domain and Fc domain (and optional hinge), with a scFv comprising a scFv variable light domain, an scFv linker and a scFv variable heavy domain. The scFv is covalently attached between the C-terminus of the CH1 domain of the heavy constant domain and the N-terminus of the first Fc domain using optional domain linkers (e.g., vh1-CH1-[optional linker]-vh2-scFv linker-vl2-[optional linker including the hinge]-CH2-CH3 or vh1-CH1-[optional linker]-vl2-scFv linker-vh2-[optional linker including the hinge]-CH2-CH3. The second monomer is a standard Fab side (e.g., vh1-CH1-[optional linker including the hinge]-CH2-CH3). This embodiment further utilizes a common light chain comprising a variable light domain (e.g., vl1) and a constant light domain, that associates with the heavy chains to form two identical Fabs that bind PSMA. As for many of the embodiments herein, these constructs include skew variants, pI variants, ablation variants, additional Fc variants, etc. as desired and described herein.

The present invention provides Central-scFv formats where the anti-CD3 scFv sequences are as shown in FIGS. 2 to 7.

The present invention provides Central-scFv formats wherein the anti-PSMA sequences are as shown in FIG. 8 and FIG. 20

The present invention provides Central-scFv formats comprising ablation variants as shown in FIG. 12.

The present invention provides Central-scFv formats comprising skew variants as shown in FIG. 10 and FIG. 15.

Central-Fv Format

One heterodimeric scaffold that finds particular use in the present invention is the Central-Fv format shown in FIG. 1. In this embodiment, the format relies on the use of an inserted scFv domain thus forming a third antigen binding domain, wherein the Fab portions of the two monomers bind PSMA and the “extra” Fv domain binds CD3. The scFv domain is inserted between the Fc domain and the CH1-Fv region of the monomers, thus providing a third antigen binding domain, wherein each monomer contains a component of the Fv (e.g., one monomer comprises a variable heavy domain and the other a variable light domain).

In this embodiment, one monomer comprises a first heavy chain comprising a first variable heavy domain, a CH1 domain and Fc domain and an additional variable light domain. The additional variable light domain is covalently attached between the C-terminus of the CH1 domain of the heavy constant domain and the N-terminus of the first Fc domain using domain linkers (e.g., vh1-CH1-[optional linker]-vl2-hinge-CH2-CH3). The other monomer comprises a first heavy chain comprising a first variable heavy domain, a CH1 domain and Fc domain and an additional variable heavy domain (e.g., vh1-CH1-[optional linker]-vh2-hinge-CH2-CH3). The additional variable heavy domain is covalently attached between the C-terminus of the CH1 domain of the heavy constant domain and the N-terminus of the first Fc domain using domain linkers.

This embodiment further utilizes a common light chain comprising a variable light domain and a constant light domain, that associates with the heavy chains to form two identical Fabs that bind PSMA. As for many of the embodiments herein, these constructs include skew variants, pI variants, ablation variants, additional Fc variants, etc. as desired and described herein.

The present invention provides Central-scFv formats where the anti-CD3 scFv sequences are as shown in FIGS. 2 to 7.

The present invention provides Central-scFv formats wherein the anti-PSMA sequences are as shown in FIG. 8 and FIG. 20.

The present invention provides Central-scFv formats comprising ablation variants as shown in FIG. 12.

The present invention provides Central-scFv formats comprising skew variants as shown in FIG. 10 and FIG. 15.

One Armed Central-scFv

One heterodimeric scaffold that finds particular use in the present invention is the one armed central-scFv format shown in FIG. 1. In this embodiment, one monomer comprises just an Fc domain, while the other monomer uses an inserted scFv domain thus forming the second antigen binding domain. In this format, either the Fab portion binds PSMA and the scFv binds CD3 or vice versa. The scFv domain is inserted between the Fc domain and the CH1-Fv region of one of the monomers.

In this embodiment, one monomer comprises a first heavy chain comprising a first variable heavy domain, a CH1 domain and Fc domain, with a scFv comprising a scFv variable light domain, an scFv linker and a scFv variable heavy domain. The scFv is covalently attached between the C-terminus of the CH1 domain of the heavy constant domain and the N-terminus of the first Fc domain using domain linkers (e.g., vh1-CH1-[optional linker]-vh2-scFv linker-vl2-[optional linker including the hinge]-CH2-CH3 or vh1-CH1-[optional linker]-vl2-scFv linker-vh2-[optional linker including the hinge]-CH2-CH3). The second monomer comprises an Fc domain (CH2-CH3). This embodiment further utilizes a light chain comprising a variable light domain and a constant light domain, that associates with the heavy chain to form a Fab. As for many of the embodiments herein, these constructs include skew variants, pI variants, ablation variants, additional Fc variants, etc. as desired and described herein.

The present invention provides one armed Central-scFv formats where the anti-CD3 scFv sequences are as shown in FIG. 2 to FIG. 7.

The present invention provides one armed Central-scFv formats wherein the anti-PSMA sequences are as shown in FIG. 8 and FIG. 20.

The present invention provides one armed Central-scFv formats comprising ablation variants as shown in FIG. 12.

The present invention provides one armed Central-scFv formats comprising skew variants as shown in FIG. 10 and FIG. 15.

Dual scFv Formats

The present invention also provides dual scFv formats as are known in the art and shown in FIG. 1. In particular, the invention provides dual scFv formats where the anti-CD3 scFv sequences are as shown in FIG. 2 to FIG. 7.

The present invention provides dual scFv formats wherein the anti-PSMA sequences are as shown in FIG. 8. In some of the embodiments, the anti-PSMA and anti-CD3 scFv includes an scFv linker. In certain embodiments, the scFv linker is an scFv linker shown in FIG. 14.

The present invention provides dual scFv formats comprising ablation variants as shown in FIG. 12.

The present invention provides dual scFv formats comprising skew variants as shown in FIG. 10 and FIG. 15.

Common Light Chain Formats

One heterodimeric scaffold that finds particular use in the present invention is the common light chain format shown in FIG. 1. In this embodiment, the first monomer comprises a first heavy chain, comprising a first variable heavy domain and a first constant heavy domain comprising a first Fc domain. The second monomer comprises a second variable heavy domain and a second constant heavy domain comprising a second Fc domain. Each of the first and second monomers are associated with a common light chain comprising a variable light domain and a constant light domain. As for many of the embodiments herein, these constructs include skew variants, pI variants, ablation variants, additional Fc variants, etc. as desired and described herein.

The present invention provides common light chain formats where the anti-CD3 sequences are as shown in FIG. 2 to FIG. 7.

The present invention provides common light chain formats wherein the anti-PSMA sequences are as shown in FIG. 8 and FIG. 20.

The present invention provides common light chain formats comprising ablation variants as shown in FIG. 12.

The present invention provides common light chain formats comprising skew variants as shown in FIG. 10 and FIG. 15.

Bispecific IgG

One heterodimeric scaffold that finds particular use in the present invention is the bispecific format shown in FIG. 1. In this embodiment, the first monomer comprises a first heavy chain, comprising a first variable heavy domain and a first constant heavy domain comprising a first Fc domain. The second monomer comprises a second variable heavy domain and a second constant heavy domain comprising a second Fc domain. Each of the first and second monomers are associated with a light chain (i.e., a first light chain and a second light chain, respectively) comprising a variable light domain and a constant light domain. As for many of the embodiments herein, these constructs include steric variants (e.g., knobs into holes variants), skew variants, pI variants, ablation variants, additional Fc variants, etc. as desired and described herein.

The present invention provides bispecific IgG formats where the anti-CD3 sequences are as shown in FIG. 2 to FIG. 7.

The present invention provides bispecific IgG formats wherein the anti-PSMA sequences are as shown in FIG. 8 and FIG. 20.

The present invention provides bispecific IgG formats comprising ablation variants as shown in FIG. 12.

The present invention provides bispecific IgG formats comprising skew variants as shown in FIG. 10 and FIG. 15.

Nucleic Acids of the Invention

The invention further provides nucleic acid compositions encoding the bispecific antibodies of the invention. As will be appreciated by those in the art, the nucleic acid compositions will depend on the format and scaffold of the heterodimeric protein. Thus, for example, when the format requires three amino acid sequences, such as for the triple F format (e.g. a first amino acid monomer comprising an Fc domain and a scFv, a second amino acid monomer comprising a heavy chain and a light chain), three nucleic acid sequences can be incorporated into one or more expression vectors for expression. Similarly, some formats (e.g. dual scFv formats such as disclosed in FIG. 1) only two nucleic acids are needed; again, they can be put into one or two expression vectors.

As is known in the art, the nucleic acids encoding the components of the invention can be incorporated into expression vectors as is known in the art, and depending on the host cells used to produce the heterodimeric antibodies of the invention. Generally the nucleic acids are operably linked to any number of regulatory elements (promoters, origin of replication, selectable markers, ribosomal binding sites, inducers, etc.). The expression vectors can be extra-chromosomal or integrating vectors.

The nucleic acids and/or expression vectors of the invention are then transformed into any number of different types of host cells as is well known in the art, including mammalian, bacterial, yeast, insect and/or fungal cells, with mammalian cells (e.g. CHO cells), finding use in many embodiments.

In some embodiments, nucleic acids encoding each monomer and the optional nucleic acid encoding a light chain, as applicable depending on the format, are each contained within a single expression vector, generally under different or the same promoter controls. In embodiments of particular use in the present invention, each of these two or three nucleic acids are contained on a different expression vector. As shown herein and in 62/025,931, hereby incorporated by reference, different vector ratios can be used to drive heterodimer formation. That is, surprisingly, while the proteins comprise first monomer:second monomer:light chains (in the case of many of the embodiments herein that have three polypeptides comprising the heterodimeric antibody) in a 1:1:2 ratio, these are not the ratios that give the best results.

The heterodimeric antibodies of the invention are made by culturing host cells comprising the expression vector(s) as is well known in the art. Once produced, traditional antibody purification steps are done, including an ion exchange chromotography step. As discussed herein, having the pIs of the two monomers differ by at least 0.5 can allow separation by ion exchange chromatography or isoelectric focusing, or other methods sensitive to isoelectric point. That is, the inclusion of pI substitutions that alter the isoelectric point (pI) of each monomer so that such that each monomer has a different pI and the heterodimer also has a distinct pI, thus facilitating isoelectric purification of the “triple F” heterodimer (e.g., anionic exchange columns, cationic exchange columns). These substitutions also aid in the determination and monitoring of any contaminating dual scFv-Fc and mAb homodimers post-purification (e.g., IEF gels, cIEF, and analytical IEX columns).

Treatments

Once made, the compositions of the invention find use in a number of applications, including the treatment of cancers that express or overexpress PSMA, including, but not limited to, prostate cancer.

Accordingly, the heterodimeric compositions of the invention find use in the treatment of these cancers.

Antibody Compositions for In Vivo Administration

Formulations of the antibodies used in accordance with the present invention are prepared for storage by mixing an antibody having the desired degree of purity with optional pharmaceutically acceptable carriers, excipients or stabilizers (Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed. [1980]), in the form of lyophilized formulations or aqueous solutions. Acceptable carriers, excipients, or stabilizers are nontoxic to recipients at the dosages and concentrations employed, and include buffers such as phosphate, citrate, and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; benzalkonium chloride, benzethonium chloride; phenol, butyl or benzyl alcohol; alkyl parabens such as methyl or propyl paraben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecular weight (less than about 10 residues) polypeptides; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugars such as sucrose, mannitol, trehalose or sorbitol; salt-forming counter-ions such as sodium; metal complexes (e.g. Zn-protein complexes); and/or non-ionic surfactants such as TWEEN™, PLURONICS™ or polyethylene glycol (PEG).

The formulation herein may also contain more than one active compound as necessary for the particular indication being treated, preferably those with complementary activities that do not adversely affect each other. For example, it may be desirable to provide antibodies with other specifcities. Alternatively, or in addition, the composition may comprise a cytotoxic agent, cytokine, growth inhibitory agent and/or small molecule antagonist. Such molecules are suitably present in combination in amounts that are effective for the purpose intended.

The active ingredients may also be entrapped in microcapsules prepared, for example, by coacervation techniques or by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsules and poly-(methylmethacylate) microcapsules, respectively, in colloidal drug delivery systems (for example, liposomes, albumin microspheres, microemulsions, nano-particles and nanocapsules) or in macroemulsions. Such techniques are disclosed in Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed. (1980).

The formulations to be used for in vivo administration should be sterile, or nearly so. This is readily accomplished by filtration through sterile filtration membranes.

Sustained-release preparations may be prepared. Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the antibody, which matrices are in the form of shaped articles, e.g. films, or microcapsules. Examples of sustained-release matrices include polyesters, hydrogels (for example, poly(2-hydroxyethyl-methacrylate), or poly(vinylalcohol)), polylactides (U.S. Pat. No. 3,773,919), copolymers of L-glutamic acid and .gamma. ethyl-L-glutamate, non-degradable ethylene-vinyl acetate, degradable lactic acid-glycolic acid copolymers such as the LUPRON DEPOT™ (injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly-D-(−)-3-hydroxybutyric acid. While polymers such as ethylene-vinyl acetate and lactic acid-glycolic acid enable release of molecules for over 100 days, certain hydrogels release proteins for shorter time periods.

When encapsulated antibodies remain in the body for a long time, they may denature or aggregate as a result of exposure to moisture at 37° C., resulting in a loss of biological activity and possible changes in immunogenicity. Rational strategies can be devised for stabilization depending on the mechanism involved. For example, if the aggregation mechanism is discovered to be intermolecular S—S bond formation through thio-disulfide interchange, stabilization may be achieved by modifying sulfhydryl residues, lyophilizing from acidic solutions, controlling moisture content, using appropriate additives, and developing specific polymer matrix compositions.

Administrative Modalities

The antibodies and chemotherapeutic agents of the invention are administered to a subject, in accord with known methods, such as intravenous administration as a bolus or by continuous infusion over a period of time, by intramuscular, intraperitoneal, intracerobrospinal, subcutaneous, intra-articular, intrasynovial, intrathecal, oral, topical, or inhalation routes. Intravenous or subcutaneous administration of the antibody is preferred.

Treatment Modalities

In the methods of the invention, therapy is used to provide a positive therapeutic response with respect to a disease or condition. By “positive therapeutic response” is intended an improvement in the disease or condition, and/or an improvement in the symptoms associated with the disease or condition. For example, a positive therapeutic response would refer to one or more of the following improvements in the disease: (1) a reduction in the number of neoplastic cells; (2) an increase in neoplastic cell death; (3) inhibition of neoplastic cell survival; (5) inhibition (i.e., slowing to some extent, preferably halting) of tumor growth; (6) an increased patient survival rate; and (7) some relief from one or more symptoms associated with the disease or condition.

Positive therapeutic responses in any given disease or condition can be determined by standardized response criteria specific to that disease or condition. Tumor response can be assessed for changes in tumor morphology (i.e., overall tumor burden, tumor size, and the like) using screening techniques such as magnetic resonance imaging (MRI) scan, x-radiographic imaging, computed tomographic (CT) scan, bone scan imaging, endoscopy, and tumor biopsy sampling including bone marrow aspiration (BMA) and counting of tumor cells in the circulation.

In addition to these positive therapeutic responses, the subject undergoing therapy may experience the beneficial effect of an improvement in the symptoms associated with the disease.

An improvement in the disease may be characterized as a complete response. By “complete response” is intended an absence of clinically detectable disease with normalization of any previously abnormal radiographic studies, bone marrow, and cerebrospinal fluid (CSF) or abnormal monoclonal protein in the case of myeloma.

Such a response may persist for at least 4 to 8 weeks, or sometimes 6 to 8 weeks, following treatment according to the methods of the invention. Alternatively, an improvement in the disease may be categorized as being a partial response. By “partial response” is intended at least about a 50% decrease in all measurable tumor burden (i.e., the number of malignant cells present in the subject, or the measured bulk of tumor masses or the quantity of abnormal monoclonal protein) in the absence of new lesions, which may persist for 4 to 8 weeks, or 6 to 8 weeks.

Treatment according to the present invention includes a “therapeutically effective amount” of the medicaments used. A “therapeutically effective amount” refers to an amount effective, at dosages and for periods of time necessary, to achieve a desired therapeutic result.

A therapeutically effective amount may vary according to factors such as the disease state, age, sex, and weight of the individual, and the ability of the medicaments to elicit a desired response in the individual. A therapeutically effective amount is also one in which any toxic or detrimental effects of the antibody or antibody portion are outweighed by the therapeutically beneficial effects.

A “therapeutically effective amount” for tumor therapy may also be measured by its ability to stabilize the progression of disease. The ability of a compound to inhibit cancer may be evaluated in an animal model system predictive of efficacy in human tumors.

Alternatively, this property of a composition may be evaluated by examining the ability of the compound to inhibit cell growth or to induce apoptosis by in vitro assays known to the skilled practitioner. A therapeutically effective amount of a therapeutic compound may decrease tumor size, or otherwise ameliorate symptoms in a subject. One of ordinary skill in the art would be able to determine such amounts based on such factors as the subject's size, the severity of the subject's symptoms, and the particular composition or route of administration selected.

Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response). For example, a single bolus may be administered, several divided doses may be administered over time or the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. Parenteral compositions may be formulated in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit contains a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.

The specification for the dosage unit forms of the present invention are dictated by and directly dependent on (a) the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and (b) the limitations inherent in the art of compounding such an active compound for the treatment of sensitivity in individuals.

The efficient dosages and the dosage regimens for the bispecific antibodies used in the present invention depend on the disease or condition to be treated and may be determined by the persons skilled in the art.

An exemplary, non-limiting range for a therapeutically effective amount of an bispecific antibody used in the present invention is about 0.1-100 mg/kg, such as about 0.1-50 mg/kg, for example about 0.1-20 mg/kg, such as about 0.1-10 mg/kg, for instance about 0.5, about such as 0.3, about 1, or about 3 mg/kg. In another embodiment, he antibody is administered in a dose of 1 mg/kg or more, such as a dose of from 1 to 20 mg/kg, e.g. a dose of from 5 to 20 mg/kg, e.g. a dose of 8 mg/kg.

A medical professional having ordinary skill in the art may readily determine and prescribe the effective amount of the pharmaceutical composition required. For example, a physician or a veterinarian could start doses of the medicament employed in the pharmaceutical composition at levels lower than that required in order to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved.

In one embodiment, the bispecific antibody is administered by infusion in a weekly dosage of from 10 to 500 mg/kg such as of from 200 to 400 mg/kg Such administration may be repeated, e.g., 1 to 8 times, such as 3 to 5 times. The administration may be performed by continuous infusion over a period of from 2 to 24 hours, such as of from 2 to 12 hours.

In one embodiment, the bispecific antibody is administered by slow continuous infusion over a long period, such as more than 24 hours, if required to reduce side effects including toxicity.

In one embodiment the bispecific antibody is administered in a weekly dosage of from 250 mg to 2000 mg, such as for example 300 mg, 500 mg, 700 mg, 1000 mg, 1500 mg or 2000 mg, for up to 8 times, such as from 4 to 6 times. The administration may be performed by continuous infusion over a period of from 2 to 24 hours, such as of from 2 to 12 hours. Such regimen may be repeated one or more times as necessary, for example, after 6 months or 12 months. The dosage may be determined or adjusted by measuring the amount of compound of the present invention in the blood upon administration by for instance taking out a biological sample and using anti-idiotypic antibodies which target the antigen binding region of the bispecific antibody.

In a further embodiment, the bispecific antibody is administered once weekly for 2 to 12 weeks, such as for 3 to 10 weeks, such as for 4 to 8 weeks.

In one embodiment, the bispecific antibody is administered by maintenance therapy, such as, e.g., once a week for a period of 6 months or more.

In one embodiment, the bispecific antibody is administered by a regimen including one infusion of an bispecific antibody followed by an infusion of an bispecific antibody conjugated to a radioisotope. The regimen may be repeated, e.g., 7 to 9 days later.

As non-limiting examples, treatment according to the present invention may be provided as a daily dosage of an antibody in an amount of about 0.1-100 mg/kg, such as 0.5, 0.9, 1.0, 1.1, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 40, 45, 50, 60, 70, 80, 90 or 100 mg/kg, per day, on at least one of day 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40, or alternatively, at least one of week 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 after initiation of treatment, or any combination thereof, using single or divided doses of every 24, 12, 8, 6, 4, or 2 hours, or any combination thereof.

In some embodiments the bispecific antibody molecule thereof is used in combination with one or more additional therapeutic agents, e.g. a chemotherapeutic agent. Non-limiting examples of DNA damaging chemotherapeutic agents include topoisomerase I inhibitors (e.g., irinotecan, topotecan, camptothecin and analogs or metabolites thereof, and doxorubicin); topoisomerase II inhibitors (e.g., etoposide, teniposide, and daunorubicin); alkylating agents (e.g., melphalan, chlorambucil, busulfan, thiotepa, ifosfamide, carmustine, lomustine, semustine, streptozocin, decarbazine, methotrexate, mitomycin C, and cyclophosphamide); DNA intercalators (e.g., cisplatin, oxaliplatin, and carboplatin); DNA intercalators and free radical generators such as bleomycin; and nucleoside mimetics (e.g., 5-fluorouracil, capecitibine, gemcitabine, fludarabine, cytarabine, mercaptopurine, thioguanine, pentostatin, and hydroxyurea).

Chemotherapeutic agents that disrupt cell replication include: paclitaxel, docetaxel, and related analogs; vincristine, vinblastin, and related analogs; thalidomide, lenalidomide, and related analogs (e.g., CC-5013 and CC-4047); protein tyrosine kinase inhibitors (e.g., imatinib mesylate and gefitinib); proteasome inhibitors (e.g., bortezomib); NF-xB inhibitors, including inhibitors of IκB kinase; antibodies which bind to proteins overexpressed in cancers and thereby downregulate cell replication (e.g., trastuzumab, rituximab, cetuximab, and bevacizumab); and other inhibitors of proteins or enzymes known to be upregulated, over-expressed or activated in cancers, the inhibition of which downregulates cell replication.

In some embodiments, the antibodies of the invention can be used prior to, concurrent with, or after treatment with Velcade® (bortezomib).

All cited references are herein expressly incorporated by reference in their entirety.

Whereas particular embodiments of the invention have been described above for purposes of illustration, it will be appreciated by those skilled in the art that numerous variations of the details may be made without departing from the invention as described in the appended claims.

EXAMPLES

Examples are provided below to illustrate the present invention. These examples are not meant to constrain the present invention to any particular application or theory of operation. For all constant region positions discussed in the present invention, numbering is according to the EU index as in Kabat (Kabat et al., 1991, Sequences of Proteins of Immunological Interest, 5th Ed., United States Public Health Service, National Institutes of Health, Bethesda, entirely incorporated by reference). Those skilled in the art of antibodies will appreciate that this convention consists of nonsequential numbering in specific regions of an immunoglobulin sequence, enabling a normalized reference to conserved positions in immunoglobulin families. Accordingly, the positions of any given immunoglobulin as defined by the EU index will not necessarily correspond to its sequential sequence.

General and specific scientific techniques are outlined in US Publications 2015/0307629, 2014/0288275 and WO2014/145806, all of which are expressly incorporated by reference in their entirety and particularly for the techniques outlined therein.

Example 1 Bispecifics Production

Amino acid sequences for anti-PSMA x anti-CD3 bispecifics are listed in FIGS. 9, and 21A-D. DNA encoding the three chains needed for bispecific expression were generated by gene synthesis (Blue Heron Biotechnology, Bothell, Wash.) and were subcloned using standard molecular biology techniques into the expression vector pTT5. Substitutions were introduced using either site-directed mutagenesis (QuikChange, Stratagene, Cedar Creek, Tex.) or additional gene synthesis and subcloning. DNA was transfected into HEK293E cells for expression and resulting proteins were purified from the supernatant using protein A affinity (GE Healthcare) and cation exchange chromatography. Cation exchange chromatography purification was performed using a HiTrap SP HP column (GE Healthcare) with a wash/equilibration buffer of 50 mM MES, pH 6.0 and an elution buffer of 50 mM MES, pH 6.0+1 M NaCl linear gradient.

The PSMA affinities (KD) for exemplary antibodies XENP14484 and XENP19722 were measured by biolayer interferometry (BLI) on an Octet Red instrument (ForteBio) (FIG. 22). Bispecifics (XENP14484, XENP19722) were captured onto an AHC sensor, followed by human or cynomolgus monkey PSMA at concentrations of 50 to 0.78125 nM (two-fold dilutions).

Example 2 Redirected T Cell Cytotoxicity

Anti-PSMA x anti-CD3 bispecifics were characterized in vitro for cell surface binding to and redirected T cell cytotoxicity (RTCC) of the PSMA⁺ LNCaP prostate cancer cell line (FIGS. 16 and 23). Assay details are indicated in the figure legend. As shown in these figures, subject anti-PSMA×anti-CD3 antibodies XENP14484,and XENP19722 were capable of binding to LNCaP prostate cancer cells and mediating RTCC killing in vitro, whereas anti-RSV control XENP13245 did not PSMA exhibit or RTCC.

Example 3 Cynomolgus Monkey Pharmacology

Cynomolgus monkeys (n=3) were given a single dose of either XENP14484 (anti-PSMA×anti-CD3, 30 μg/kg) or XENP13245 (anti-RSV×CD3, 3 mg/kg). CD4⁺ T cell margination and activation by CD69 MFI were examined by flow cytometry. IL-6 serum levels were examined by ELISA (FIG. 17A). In addition, CD8⁺ T cell margination and activation by CD69 MFI were examined by flow cytometry and TNFα serum levels were examined by ELISA (FIG. 17B). As shown in these figures, T cell response and Cytokine Release Syndrome (CRS) is dependent on the engagement of target cells by the anti-PSMA×anti-CD3 antibody in monkeys.

Example 4 CD3 Affinity and Cytokine Release Syndrome

Cynomolgus monkey Cynomolgus monkeys (n=3) were administered a single intraveneous (i.v.) dose of either 0.06 mg/kg XENP14484 (high CD3 affinity) or 1 mg/kg XENP19722 (low CD3 affinity). As shown in FIG. 24, XENP14484 and XENP19722 antibodies exhibit similar T cell margination and activation (CD4+ (top panel) and CD8+ (middle panel)) despite having different CD3 affinities. Despite similarities in T cell activation, however, XENP19722, which has a low affinity to CD3, induced less cytokine reduced syndrome (CRS) compared to XENP14484, which has a high affinity to CD3 (FIG. 24, bottom panel). Such lower CD3 affinity correlates with reduced cytokine release even at higher dose levels. As such, the potency of anti-PSMA bispecifics can be modulated by tuning their ability to recruit T cells. 

1-55. (canceled)
 56. A composition comprising a prostate membrane antigen binding domain (PSMA) binding domain, said PSMA binding domain comprising: a) a heavy variable domain having amino acid sequence of SEQ ID NO: 110; and b) a variable light domain having amino acid sequence of SEQ ID NO: 106
 57. A heterodimeric antibody comprising: a) a first monomer comprising: i) a first Fc domain; and ii) an anti-CD3 scFv comprising a first heavy variable domain, an scFv linker and a first variable light domain; wherein said scFv is covalently attached to the N-terminus of said Fc domain using a domain linker; b) a second monomer comprising a heavy chain comprising: i) a second heavy variable domain; and ii) a heavy constant domain comprising a second Fc domain; and c) a light chain comprising a second variable light domain and a constant light domain; wherein said second heavy variable domain comprises a vhCDR1 having SEQ ID NO: 111, a vhCDR2 having SEQ ID NO: 112, and a vhCDR3 having SEQ ID NO: 113, and said second variable light domain comprises a vlCDR1 having SEQ ID NO: 107, a vlCDR2 having SEQ ID NO: 108, and a vlCDR3 having SEQ ID NO: 109, and wherein said second heavy variable domain and said second variable light domain bind PSMA.
 58. A heterodimeric antibody according to claim 57, where said second heavy variable domain comprises amino acid sequence SEQ ID NO: 110 and said second variable light domain comprises amino acid sequence SEQ ID NO:
 106. 59. A heterodimeric antibody according to claim 57, wherein said first variable heavy domain comprises SEQ ID NO: 1 and said first variable light domain comprises SEQ ID NO:
 5. 60. A heterodimeric antibody according to claim 59, wherein said first monomer comprises SEQ ID NO: 64, said second monomer comprises SEQ ID NO: 65, and said light chain comprises SEQ ID NO:
 66. 61. A heterodimeric antibody according to claim 57, wherein said first variable heavy domain comprises SEQ ID NO: 10 and said first variable light domain comprises SEQ ID NO:
 14. 62. A heterodimeric antibody according to claim 61, wherein said first monomer comprises SEQ ID NO: 124, said second monomer comprises SEQ ID NO: 125, and said light chain comprises SEQ ID NO:
 126. 63. A heterodimeric antibody according to claim 57, wherein said first variable heavy domain comprises SEQ ID NO: 37 and said first variable light domain comprises SEQ ID NO:
 41. 64. A heterodimeric antibody according to claim 63, wherein said first monomer comprises SEQ ID NO: 118, said second monomer comprises SEQ ID NO: 119, and said light chain comprises SEQ ID NO:
 120. 65. A heterodimeric antibody according to claim 57, wherein said first variable heavy domain comprises SEQ ID NO: 46 and said first variable light domain comprises SEQ ID NO:
 50. 66. A heterodimeric antibody according to claim 65, wherein said first monomer comprises SEQ ID NO: 121, said second monomer comprises SEQ ID NO: 122, and said light chain comprises SEQ ID NO:
 123. 67. A heterodimeric antibody according to claim 57, wherein said first variable heavy domain comprises SEQ ID NO: 19 and said first variable light domain comprises SEQ ID NO:
 23. 68. A heterodimeric antibody according to claim 57, wherein said first variable heavy domain comprises SEQ ID NO: 28 and said first variable light domain comprises SEQ ID NO:
 32. 69. A heterodimeric antibody according to claim 57, wherein said first Fc domain and said second Fc domain comprise a set of variants selected from the group consisting of S364K/E357Q: L368D/K370S; L368D/K370S: S364K; L368E/K370S: S364K; T411T/E360E/Q362E: D401K; L368D/K370S: S364K/E357L and K370S: S364K/E357Q.
 70. A heterodimeric antibody according to claim 57, wherein said scFv linker is a charged linker.
 71. A heterodimeric antibody according to claim 57, wherein said heavy chain constant domain comprises the amino acid substitutions N208D/Q295E/N384D/Q418E/N421D.
 72. A heterodimeric antibody according to claim 57, wherein said first and second Fc domains comprise the amino acid substitutions E233P/L234V/L235A/G236del/S267K.
 73. A nucleic acid composition encoding the heterodimeric antibody according to claim 57, said composition comprising: a) a first nucleic acid encoding said first monomer; b) a second nucleic acid encoding said second monomer; and c) a third nucleic acid encoding said light chain.
 74. An expression vector composition comprising the nucleic acid composition of claim
 73. 74. A host cell comprising the nucleic acid composition of claim
 73. 75. A host cell comprising the expression vector composition of claim
 74. 76. A method of making a heterodimeric antibody according to any previous claim comprising culturing the host cell of claim 74 or 75 under conditions wherein said antibody is expressed, and recovering said antibody.
 77. A method of treating a cancer in a patient in need thereof comprising administering a heterodimeric antibody according to claim 57 to said patient. 